Effect of resveratrol on inhibiting inflammatory responses of astrocytes and protecting neurons from injury in cerebral ischemic mice
10.3760/cma.j.issn.1671-8925.2018.08.002
- VernacularTitle:白藜芦醇对脑缺血损伤后星形胶质细胞炎症反应的影响及其神经保护作用研究
- Author:
Jingjing ZHAO
1
;
Zhenkun YANG
;
Jie SUN
;
Wei WANG
;
Zaiwang LI
;
Jingyu CHEN
Author Information
1. 南京医科大学附属无锡人民医院临床研究中心
- Keywords:
Resveratrol;
Cerebral ischemic injury;
Astrocyte;
Inflammation;
Neuroprotection
- From:
Chinese Journal of Neuromedicine
2018;17(8):765-771
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of resveratrol on inhibiting inflammatory responses of astrocytes and protecting neurons from injury in cerebral ischemic mice.Methods (1) Sixty adult male C57/BL6 mice were randomly assigned to sham-operated group,ischemic group (mice were subjected to transient global ischemia by bilateral common carotid artery occlusion after injection of 1.5％ DMSO),and resveratrol treatment group (mice were subjected to transient global ischemia after injection of 20 mg/kg resveratrol dissolved in 1.5％ DMSO),with 20 mice in each group;the neuronal damage in the ischemic mouse brains was analyzed by Cresyl Violet (CV) staining.(2) In vitro,the primary cultured astrocytes were divided into oxygen-glucose deprivation (OGD)-treated astrocytes group (DMSO was added into the culture medium of astrocytes followed by OGD treatment) and resveratrol-treated astrocytes group (DMSO and 25 μmol/L resveratrol were added into the culture medium of astrocytes followed by OGD treatment);the expressions of tumor necrosis factor (TNF)-α and interleukin (IL)-6 were analyzed by ELISA at re-oxygenation 12 h after 4 h-OGD treatment;meanwhile,the primary cultured neurons were injured by OGD treatment,and subsequently were divided into OGD-treated neurons group (neurons were incubated with culture medium of OGD-treated astrocytes) and resveratrol-treated neurons group (neurons were incubated with culture medium of resveratrol-trcated astrocytes).The neurite outgrowth and expressions of TNF-α and IL-6 in astrocytes of each group were analyzed by immunofluoresent staining,and the lactic dehydrogenase (LDH) release of OGD injured neurons was detected by LDH Kit.Results (1) CV staining showed that the degrees of hippocampal neurons injury in the resveratrol treatment group (1.50±0.34) were significantly decreased as compared with those in the ischemic group (3.17±0.31,P＜0.05).(2) ELISA showed that the expressions of TNF-α and IL-6 in the resveratrol-treated astrocytes group ([160.2±18.41] pg/mL,[241.25±42.22] pg/mL) were significantly decreased as compared with those in the OGD-treated astrocytes group ([282.4 ±17.01] pg/mL,[550.5 ±59.02] pg/mL,P＜0.05);LDH detection showed the release of LDH from the resveratrol-treated neurons (0.30±0.05) was dramatically decreased as compared with that from the OGD-treated neurons (0.62±0.08,P＜0.05);immunofluorescence staining showed that the TNF-α and lL-6 positive expressions in the resveratrol-treated astrocytes were obviously decreased as compared with those in the OGD-treated astrocytes,and the expressions of neurites of the resveratrol-treated neurons were obviously increased as compared with those in the OGD-treated neurons.Conclusion Resveratrol has neuroprotective effect on neurons in global cerebral ischemic mice,probably via down-regulating the expressions and secretion of inflammatory cytokines TNF-α and IL-6 from the astrocytes.
