Influence of spinal cord injury in hypothalamus AMP-activated protein kinase expression and glucose level
10.3760/cma.j.issn.1671-8925.2018.07.004
- VernacularTitle:脊髓损伤对下丘脑AMPK通路及血糖水平的影响
- Author:
Ruidong CHENG
1
;
Liying HUANG
;
Ting YANG
;
Peng SUN
;
Qi LI
;
Li ZHANG
;
Juebao LI
;
Xiangming YE
Author Information
1. 浙江省人民医院、杭州医学院附属人民医院康复医学科
- Keywords:
Spinal cord injury;
Hypothalamus;
AMP-activated protein kinase;
Insulin resistance
- From:
Chinese Journal of Neuromedicine
2018;17(7):668-672
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the influence of spinal cord injury (SCI) in hypothalamus AMP-activated protein kinase (AMPK) expression,and explore the mechanism of SCI-induced type 2 diabetes or insulin resistance.Methods Eighteen SD rats were randomly divided into model group,High Fat/High Sucrose/High Cholesterol Diet group and SCI group (n=6).The animals in the model group and High Fat/High Sucrose/High Cholesterol Diet group underwent a T10 laminectomy without weight-drop injury,and SCI models were induced by MASCIS Impactor method.Blood glucose and glucose tolerance tests were performed before experiment and 8 weeks after the experiment.At 8 weeks,the levels of serum inflammatory factors,tumor necrosis factor (TNF)-α,interleukin (IL)-1β,and IL-6,were observed by ELISA;Western blotting was used to detect the expression of phosphorylated (p) -AMPK in the hypothalamus.Results Blood glucose levels in the rats of High Fat/High Sucrose/High Cholesterol Diet group and SCI group were significantly higher than that in the model group (P＜0.05).Blood glucose levels 15 and 60 min after SCI in rats of SCI group were significantly higher than those in the model group (P＜0.05);Blood glucose levels 15,30,60 and 120 min after SCI in rats of High Fat/High Sucrose/High Cholesterol Diet group were significantly higher than those in the model group (P＜0.05).As compared with those in the model group,the expression of p-AMPK in the hypothalamus were significantly decreased,and the TNF-α,IL-1β and IL-6 levels were statistically increased in rats of High Fat/High Sucrose/High Cholesterol Diet group and SCI group (P＜0.05).Conclusion SCI could inhibit AMPK expression in the hypothalamus,activate inflammatory signaling pathway and enhance chronic systemic inflammation,and therefore,insulin resistance is induced.
