Immunomodulatory Effects of Mesenchymal Stem Cells in a Murine Model of TNBS-Induced Colitis.
10.4174/jkss.2010.79.5.317
- Author:
Yong Beom CHO
1
;
Min Shik KIM
;
Min Jeong KANG
;
Hee Jung SHIN
;
Seok Hyung KIM
;
Hee Cheol KIM
;
Seong Hyeon YUN
;
Woo Yong LEE
;
Ho Kyung CHUN
Author Information
1. Department of Surgery, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea. hkchun@skku.edu
- Publication Type:Original Article
- Keywords:
Mesenchymal stem cell;
Inflammatory bowel disease;
Crohn's disease
- MeSH:
Administration, Rectal;
Animals;
B-Lymphocytes;
Colitis;
Crohn Disease;
Cytokines;
Ethanol;
Humans;
Inflammation;
Inflammatory Bowel Diseases;
Interleukin-10;
Interleukin-12;
Interleukin-4;
Interleukin-6;
Mesenchymal Stromal Cells;
Mice;
Regeneration;
Spleen;
Tumor Necrosis Factor-alpha;
Wasting Syndrome
- From:Journal of the Korean Surgical Society
2010;79(5):317-325
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: Mesenchymal stem cells (MSCs) are surfacing as a new method of treatment for various diseases that have poor outcome with drug treatments. In this study, we investigated the effects of MSCs in a murine intestinal inflammation model mimicking human Crohn's disease (CD) using 2,4,5-trinitrobenzene sulfonic acid (TNBS). METHODS: Colitis was induced by rectal administration of 2 mg of TNBS in 35% ethanol as experimental group compared to control group. Histological changes, surface molecules of T and B cells of the spleen and blood, and cytokine production (IFN-gamma, TNF-alpha, IL-4, IL-6, IL-10, and IL-12) were determined among 3 groups comprised of control group, TNBS group and TNBS/MSC group. RESULTS: In the mice treated with MSCs, there was a decrease in the wasting disease process and inflammatory histopathological changes. There was also a decrease in pro-inflammatory T-helper 1 (Th1) cytokines IFN-gamma and IL-12 and T-helper 2 (Th2) cytokine IL-4. Anti-inflammatory cytokine IL-10 increased in mice treated with MSCs compared to colitic mice. The blood CD4+CD25+ T-regulatory cells also increased and splenic CD19 B-cells decreased. CONCLUSION: The results of this study suggest that MSCs may have a therapeutic effect in controlling the Th1 and Th2 mediated immune response in patients with CD and aid in tissue regeneration.
