Effect of apolipoprotein E on secretions of matrix metalloproteinase-9, matrix metalloproteinase tissue inhibitor-1 and inflammatory factors in macrophages at early stage of experimental autoimmune encephalomyelitis
10.3760/cma.j.issn.1671-8925.2018.02.006
- VernacularTitle:载脂蛋白E对EAE小鼠发病前期巨噬细胞分泌MMP-9、 TIMP-1及炎症因子的影响
- Author:
Zhenmei LU
1
;
Wenyu LU
;
Yulan TANG
;
Lijun FANG
;
Yifeng LI
;
Junjie WEI
;
Yunfei WEI
Author Information
1. 广西医科大学第一附属医院神经内科
- Keywords:
Apolipoprotein E;
Experimental autoimmune encephalomyelitis;
Macrophage;
Matrix metalloproteinase-9;
Matrix metalloproteinase tissue inhibitor-1;
Inflammatory factor
- From:
Chinese Journal of Neuromedicine
2018;17(2):136-141
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of apolipoprotein E (ApoE) on secretions of matrix metalloproteinase-9 (MMP-9),matrix metalloproteinase tissue inhibitor-1 (TIMP-1) and inflammatory factors in spleen macrophages at early stage of experimental autoimmune encephalomyelitis (EAE).Methods In accordance with random number table,20 ApoE knockout (ApoE-/-) C57BL/6J mice were divided into ApoE-/-EAE group and ApoE-/-control group (n=10);and 20 wild type (WT) C57BL/6J mice were divided into WT EAE group and WT control group (n=10).Myelin oligodendrocyte glycoprotein 35-55 (MOG35-55) was used as antigen to induce EAE models in the ApoE-/-EAE group and WT EAE group,and MOG35-55 was replaced by normal saline in ApoE-/-control group and WT control group.The spleen macrophages were isolated from mice after 7th d of modeling and cultured in vitro;the rnacrophages of the ApoE-/-EAE group and WT EAE group were stimulated by MOG35-55,while the macrophages of the ApoE-/-control group and WT control group were treated with equivalent normal saline.Forty-eight hafter that,the contents of MMP-9,TIMP-1,interferon gamma-γ(IFN-γ),tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the culture medium were determined by ELISA.Results The contents of MMP-9,TFN-γ TNF-α and IL-6 in the WT EAE group were significantly higher than those in the WT control group (P＜0.05);the contents of MMP-9,IFN-γ 、TNF-α and IL-6 in the ApoE-/-EAE group were significantly higher than those in the ApoE-/-control group (P＜0.05);the content ofMMP-9,IFN-γ,TNF-cα and IL-6 in ApoE-/-EAE group were significantly higher than those in the WT EAE group (P＜0.05).The TIMP-1 secretion ofmacrophages in WT EAE group was significantly higher than that in WT control group (P＜0.05).Conclusions At early stage of EAE,macrophages secrete high levels of MMP-9 and inflammatory factors,and the deficiency of ApoE further contributes to the secretions of MMP-9 and inflammatory factors by macrophages.ApoE may play a role in protecting the integrity of blood-brain barrier and alleviating the inflammatory response of the central nervous system through the effects on macrophages.
