Shuxuetong promotes collateral circulation formation and cerebrovascular reserve capacity in mouse models after ischemic stroke
10.3760/cma.j.issn.1671-8925.2018.01.002
- VernacularTitle:疏血通对脑梗死小鼠侧支循环形成及脑血管储备能力的影响
- Author:
Jia YANG
1
;
Zhandong QIU
;
Gang DENG
;
Yu FANG
;
Dayong LI
;
Suming ZHANG
Author Information
1. 首都医科大学附属北京友谊医院神经内科
- Keywords:
Shuxuetong;
Ischemic stroke;
Angiogenesis;
Collateral circulation;
Cerebrovascular reserve capacity
- From:
Chinese Journal of Neuromedicine
2018;17(1):10-18
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Shuxuetong (SXT) on promoting collateral circulation formation and enhancing cerebrovascular reserve capacity in mouse models after ischemic stroke.Methods Fifty-eight C57BL/6 mice were randomly divided into blank control group (n=6),SXT group (n=26) and control group (n=26).The mice in the SXT group and control group were subjected to distal middle cerebral artery occlusion (dMCAO) by electrocoagulation,and intraperitoneal administration of SXT (1.5 mL/kg) or equal volume of saline was given 30 min after stroke and repeated every 12 h for the next 3 d.TTC staining was used to assess the volume of infarction one d after ischemic stroke.Adhesive removal tests were performed to evaluate sensorimotor function of the mice before and one,3,7,14,21 and 28 d after stroke,Standardized regional cerebral blood flow (SrCBF) was measured by laser speckle contrast imaging before and one,3,and 7 d after stroke,Immunofluorescence was used to evaluate the numbers of deoxyuridine 5'-monophosphate disodium (BrdU)+,BrdU+/von Willebrand factor (vWF)+ and BrdU+/Laminin+ cells and vessel density 7 d after stroke.The expression levels of total protein kinase (AKT),phosphorylated (p)-AKT,vascular endothelial growth factor (VEGF) and myeloperoxidase (MPO) were explored by Western blotting one d after stroke.Results As compared with those in the control group,the infarction volume of SXT group was significantly smaller one d after stroke,SrCBF was significantly higher 3 and 7 d after stroke,and vessel density was significantly increased and numbers of BrdU+/vWF+ and BrdU+/laminin+ cells in the subventricular zone and peri-infarction region were statistically larger 7 d after stroke (P＜0.05).The results of SXT group in behavior experiments were significantly better than those of control group one d after stroke (P＜0.05).The levels of p-AKT and VEGF of SXT group were significantly up-regulated,while MPO level was significantly decreased in peri-infarction tissues as compared with those in the control group one d after stroke (P＜0.05).Conclusion SXT can promote the formation of collateral circulation and enhance the cerebrovascular reserve canacity.
