Effect of ulinastatin on neuron apoptosis and CCAAT/enhancer-binding protein expression of spinal cord after peripheral nerve injury
10.3760/cma.j.issn.1671-8925.2017.11.012
- VernacularTitle:乌司他丁对周围神经损伤后脊髓神经元凋亡及CCAAT增强子结合蛋白表达的影响
- Author:
Jia LI
1
;
Jianwei WANG
;
Meng WANG
;
Fang XING
Author Information
1. 郑州大学附属肿瘤医院麻醉科
- Keywords:
Ulinastatin;
CCAAT/enhancer-binding protein;
Apoptosis;
Peripheral nerve;
Spinal cord
- From:
Chinese Journal of Neuromedicine
2017;16(11):1139-1146
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of ulinastatin on neuron apoptosis and CCAAT/enhancer-binding protein (CHOP) expression of spinal cord after peripheral nerve injury.Methods A total of 225 healthy male SPF C57BL/6J mice were divided into three groups by using a random number table:sham-operated group,peripheral nerve injury group and ulinastatin group (n=75).The models of unilateral sciatic nerve transection were established in the latter two groups.After the models being established,intraperitoneal injection ofulinastatin 0.2 mL (10 000 U/kg) was performed once daily for 3 consecutive d in ulinastatin group,and the equal volume of normal saline was given once daily for 3 consecutive d in sham-operated group and peripheral nerve injury group.One,3,7,14 and 28 d after surgery,L4-6 spinal cord segments were removed for pathological examination by HE staining,and for detection of neuron apoptosis and apoptotic index (AI) by TUNEL method;the expressions of CHOP,Bcl-2,Bax and cleaved caspase-3 proteins were determined by Western blotting and the ratio of Bcl-2/Bax was calculated,and the CHOP mRNA expression was detected by RT-PCR.Results HE staining showed that the injury of spinal cord in peripheral nerve injury group was more aggravated as compared with that in the sham-operated group,and the injury of spinal cord in ulinastatin group was more alleviated as compared with that in the peripheral nerve injury group.One,3,7,14 and 28 d after surgery,AI was significantly higher,Bcl-2 protein expression was down-regulated,cleaved caspase-3 and Bax protein expressions were up-regulated,Bcl-2/Bax ratio was lower,and CHOP protein or mRNA expressions were up-regulated in the peripheral nerve injury group and ulinastatin group as compared with those in the sham-operated group,with statistically significant differences (P＜0.05).As compared with those in the peripheral nerve injury group,AI was significantly lower,Bcl-2 protein expression was up-regulated,cleaved caspase-3 and Bax protein expressions were down-regulated,Bcl-2/Bax ratio was higher,and CHOP protein or mRNA expressions were down-regulated in ulinastatin group,with statistically significant differences (P＜0.05).Conclusion The mechanism by which ulinastatin protects spinal cord injury after peripheral nerve injury is related to down-regulation of CHOP expression and suppression of neuron apoptosis of spinal cord.
