Bevacizumab alleviates damage of capillaries and blood-brain barrier in whole-brain irradiation mice
10.3760/cma.j.issn.1671-8925.2017.10.009
- VernacularTitle:贝伐珠单抗可减轻X线全脑照射小鼠脑血管及血脑屏障损伤
- Author:
Junjie GUO
1
;
Haiwei HUANG
;
Guoyong HE
;
Ying XIE
Author Information
1. 中山大学第一附属医院神经内科
- Keywords:
Bevacizumab;
Radiation-induced brain injury;
Cerebral vessel;
Blood brain barrier
- From:
Chinese Journal of Neuromedicine
2017;16(10):1016-1021
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of bevacizumab (BVZ) on cerebral vessel and blood brain barrier changes in whole-brain irradiation mice.Methods Seventy-five male mice aged 6-7 weeks were randomly divided into normal control group,BVZ group and radiation group (n=25).Mice firom normal control group were without whole-brain irradiation or drug administration.Mice from BVZ group and radiation group were accepted fractionated cranial irradiation at a total dose of 30 Gy by X-ray.Mice from BVZ group and radiation group were intraperitoneally injected with 10 mg/kg BVZ solution or equivalent normal saline (10 weeks continuously) at the beginning of the first X-ray whole-brain irradiation.Eleven weeks after the first irradiation,HE staining was used to observe cerebrovascular pathological changes;Western blotting was used to test vascular endothelial growth factor (VEGF) protein expression;Evan Blue (EB) staining was used to assess the blood-brain barrier damage,and immunofluorescence staining was used to test the yon willebrand factor (vWF) and tight junction protein-1 (ZO-1) expressions.Results The VEGF protein expression level and brain tissue EB content ([9.66±0.73] μg/mL) in the normal control group were significantly increased as compared with those in the radiation group and BVZ group ([16.90±2.15] μg/mL and [12.29±0.96] μg/mL,P＜0.05),but ZO-1/vWF ratio (0.92±0.08) in normal control group was significantly decreased as compared with that in the radiation group and BVZ group (0.46±0.18 and 0.75±0.11,P＜0.05).As compared with the radiation group,BVZ group had significantly reduced VEGF protein expression and EB level in the brain tissues,and had significantly increased ZO-1/vWF ratio (P＜0.05).Conclusion BVZ decreases the quantity of VEGF in brain tissues and alleviates the continuous damage of the brain vessels and blood brain barrier in whole-brain irradiation mice.
