Role of NT-3 gene modified mesenchymal stem cells and its mechanism in treatment of acute cerebral infarction
10.3760/cma.j.issn.1671-8925.2017.09.008
- VernacularTitle:NT-3基因修饰骨髓间充质干细胞移植治疗脑梗死及其机制研究
- Author:
Hetao BIAN
1
;
Lou HAO
;
Wei HUANG
Author Information
1. 济宁市第一人民医院神经内科
- Keywords:
Bone marrow mesenchymal stem cell;
Neurotrophin-3;
Gene modification;
Stem cell transplantation;
Cerebral ischemia
- From:
Chinese Journal of Neuromedicine
2017;16(9):906-910
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of neurotrophin-3 (NT-3) gene modified bone marrow mesenchymal stem cells (BMSCs) in treatment of acute cerebral infarction and its underlying mechanism.Methods Seventy-two SD male rats were randomly divided into model group,BMSCs group,and BMSCs+NT-3 group (n=24).Focal cerebral ischemia rat models were established by middle cerebral artery occlusion (MCAO);24 h after MCAO,rats in the BMSCs group and BMSCs+NT-3 group were given BMSCs and NT-3 gene modified BMSCs via tail intravenous injection,respectively.Modified neurological severity scale (mNSS) was performed one,6,12 and 24 d after MCAO;infarct sizes were measured by TTC staining one,12 and 24 d after MCAO;expressions of neuronspecific enolase (NSE) and nestin in the surrounding areas of infarction were detected by Western blotting 6 d after MCAO.Results As compared with those in the BMSCs group,significantly decreased mNSS scores in BMSCs+NT-3 group were noted 6,12 and 24 d after MCAO (P＜0.05).Rats in the BMSCs+NT-3 group had significantly smaller brain infarct sizes than the BMSCs group 12 and 24 d after MCAO (P＜0.05).Expressions of NSE and nestin in the BMSCs+NT-3 group were significantly higher than those in the BMSCs group 6 d after MCAO (P＜0.05).Conclusion Transplantation of NT-3 gene modified BMSCs can further improve neurological functions and reduce brain infarct sizes as compared with BMSCs transplantation,whose mechanism might be related to promote the differentiation of BMSCs into neurons.
