Effects of two methods of nimodipine administration on mitochondrial pathway in the hippocampus of rabbit models of subarachnoid hemorrhage
10.3760/cma.j.issn.1671-8925.2017.08.006
- VernacularTitle:尼莫地平2种给药途径对兔蛛网膜下腔出血后海马线粒体通路的影响
- Author:
Hao DING
1
;
Jin CHEN
;
Yongjian FU
;
Songsong ZHANG
;
Xianqing SHI
Author Information
1. 贵州省骨科医院重症医学科
- Keywords:
Nimodipine;
Administration method;
Subarachnoid hemorrhage;
Mitochondrial pathway;
Apoptosis
- From:
Chinese Journal of Neuromedicine
2017;16(8):790-795
- CountryChina
- Language:Chinese
-
Abstract:
Objective To compare the effects of two methods of nimodipine administration on mitochondrial pathway in the hippocampus of rabbit models of subarachnoid hemorrhage.Methods Twenty-four New Zealand white rabbits were randomly allocated to sham-operated group,subarachnoid hemorrhage (SAH) group and nimodipine introvenous injection (ND1) group,and nimodipine intracistemal administration (ND2) group (n=6).All animals underwent operation under anaesthesia;one mL/kg autologous nonheparinized arterial blood was injected into cisterna magna in SAH group,ND1 and ND2 group,and one mL/kg saline into cisterna magna in sham-operated group.Thirty minutes after SAH,0.5 mL/kg 0.2％ nimodipine was injected into cisterna magna in ND2 group,and equal-volume saline into cisterna magna in sham-operated group,SAH group and ND1 group.While 0.5 mL/kg 0.2％ nimodipine via introvenously injection was performed in the ND1 group,and equal-volume saline via introvenously injection into the sham-operated group,SAH group and ND2 group.All animals were assessed for the grading of food intake and neurological impairment 72 h after SAH,and then,they were scarified;their hippocampi were processed for detecting the mRNA and protein expressions of Caspase-3 and Cyt-c by using real time-PCR and Western blotting.Results The differences of food intake and neurological impairment between the four groups were statistically significant 72 h after SAH (H=16.664,P=0.001;H=15.411,P=0.001);according mean rank,the food intake and neurological impairment in the ND2 group were decreased as compared with those in the ND2 group.The mRNA and protein expression levels of Caspase-3 and Cyt-c among the four groups were statistically different (P＜0.05).As compared with those in the sham-operated group and SAH group,the mRNA and protein expression levels of Caspase-3 and Cyt-c were significantly higher in the ND1 and ND2 groups (P＜0.05);As compared with ND1 group,ND2 group had significantly lower mRNA and protein expression levels of Caspase-3 and Cyt-c (P＜0.05).Conclusion Intracistemal administration ofnimodipine could decrease mRNA and protein expressions of Caspase-3 and Cyt-c and inhibit activation of mitochondrial pathway in hippocampus in rabbit models of SAH.
