Hyperbaric oxygen combined with bone marrow mesenchymal stem cells transplantation reduced inflammatory reaction in rats with spinal cord injury and its relation with nuclear factor-kappa B signal pathway inhibition
10.3760/cma.j.issn.1671-8925.2017.08.004
- VernacularTitle:HBO联合BMSCs移植减轻脊髓损伤大鼠炎症反应与NF-κB炎症信号通路有关
- Author:
Yuqin HUANG
1
;
Meng LI
;
Xiaojing ZHANG
;
Hui ZHANG
;
Yinhai CHEN
Author Information
1. 南方医科大学珠江医院神经康复及高压氧科
- Keywords:
Spinal cord injury;
Hyperbaric oxygen;
Bone marrow mesenchymal stem cell;
Nuclear factor-kappa B;
Inflammatory reaction
- From:
Chinese Journal of Neuromedicine
2017;16(8):777-784
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism of hyperbaric oxygen (HBO) combined with bone marrow mesenchymal stem cells (BMSCs) transplantation to reduce the inflammatory response of spinal cord injury (SCI) in rats,and whether it is related to nuclear factor-kappa B (NF-κB) signaling pathway or not.Methods One hundred and sixty male adult SD rats were randomly divided into 4 groups according to the random number table:sham-operated group,SCI group,BMSCs transplantation group and BMSCs transplantation+HBO group (n=40).The rats from the latter three groups were established the SCI models by modified Allen dropping;and 1 mL BMSCs (3 × 106 cells) suspension was given by tail vein injection one h after SCI into the rats of the BMSCs transplantation group and BMSCs transplantation+HBO group;rats in the BMSCs transplantation+HBO group were given hyperbaric oxygen treatment.Rats were evaluated for locomotor function of hind limbs with Basso-Beattle-Bresnahan (BBB) scale one,3,7,14 d after SCI.Three d after SCI,the expression levels of NF-κB,IκB-α,tumor necrosis factor (TNF)-α and interleukin (IL)-1β in the spinal cord tissues were determined by Westem blotting;and enzyme-linked immuno sorbent assay (ELISA) was employed to detect the TNF-α and IL-1β levels.The number of NF-κB p65 positive cells in the spinal cord tissues was determined by immunohistochemistry.The histopathological changes of injured spinal cords were observed by HE staining.Results BBB scores of BMSCs transplantation group and BMSCs transplantation+HBO group were significantly higher than those of SCI group 3,7 and 14 d after SCI (P＜0.05),and those in the BMSCs transplantation+HBO group increased more significantly.The contents of NF-κB,TNF-α and IL-1β in the spinal cord tissues of BMSCs transplantation group were significantly lower than those in the SCI group 3 d after SCI,and the content of IκB-α protein was significantly increased (P＜0.05);the contents of NF-κB,TNF-α and IL-1β in the spinal cord tissues of BMSCs transplantation+HBO group were significantly lower than those in the BMSCs transplantation group,and the content of IκB-α protein was significantly increased (P＜0.05).The concentrations of TNF-α and IL-1β in the spinal cord tissues of BMSCs transplantation group were significantly lower than those in the SCI group (P＜0.05);the concentrations of TNF-α and IL-1β in the BMSCs transplantation+HBO group were significantly lower than those in BMSCs transplantation group (P＜0.05).The number of NF-κB p65 positive cells in the BMSC transplantation group was significantly smaller than that in SCI group (P＜ 0.05);the number of NF-κB p65 positive cells in BMSCs transplantation+HBO group was significantly larger than that in the BMSCs transplantation group (P＜0.05).As compared with those in the SCI group,the inflammatory response and edema were reduced,the area of spinal cord empty area decreased,and the damage area was improved in the BMSCs transplantation group and BMSCs transplantation+HBO group.Conclusion HBO combined with BMSCs transplantation has a synergistic effect after SCI,which might be partially due to down regulation of signal pathway of NF-κB and reduce of inflammatory reaction,and it might play a role in the treatment of spinal cord secondary injury.
