Hemopexin protects endothelial integrity by promoting endothelial progenitor cells differentiation and new blood vessels formation through heme oxygenase-1 pathway
10.3760/cma.j.issn.1671-8925.2017.08.002
- VernacularTitle:血红素结合蛋白通过上调HO-1表达促进EPCs分化以修复血管内皮损伤
- Author:
Yongyan YANG
1
;
Beibei DONG
;
Guolin WANG
;
Yonghao YU
Author Information
1. 天津医科大学总医院麻醉科
- Keywords:
Hemopexin;
Heme oxygenase-1;
Endothelial progenitor cell
- From:
Chinese Journal of Neuromedicine
2017;16(8):766-771
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of hemopexin (HPX) on promoting endothelial progenitor cells (EPCs) differentiation and neovascularization to protect endothelial integrity from toxic heme through heme oxygenase-1 (HO-1) pathway.Methods Rat EPCs were cultured routinely,and randomly divided into 5 groups (at their logarithmic phase):control group,HPX group,heme group,heme+HPX group,and Zn protoporphyrin Ⅸ (ZnppⅨ) group.Cells in the control group were cultured in M199 culture medium with 10％ fetal calf serum,5 μg/mL basic fibroblast growth factor and 1％ penicillin-streptomycin,and 20 μmol/L HPX,20 μmol/L heme,20 μmol/L heme-HPX compound,and 20 μmol/L heme-HPX compound and 10 μmol/L ZnppⅨ were added in the culture mediums of HPX group,heme group,heme+HPX group and ZnppⅨ group,respectively.Cells were harvested after 24 h of culture;HO-1 expression was detected by Western blotting;differentiation of EPCs to mature endothelial cells was investigated using von Willebrand factor (vWF) immunofluorescence;vasculogenic ability was tested using angiogenesis assay on Matrigel Basement Membrane Matirx.Results As compared with that in the control group (0.60±0.15),HO-1 expression increased significantly in heme group (1.07±0.03,P＜0.05);as compared with the heme group,the HO-1 expression in the heme+HPX group (1.57±0.06) significantly increased (P＜0.05).As compared with those in the control group,the EPCs number was smaller,CD31 and vWF expressions were decreased and immunofluorescence results were weaker in the heme group;as compared with those in the control group,the EPCs number was larger,CD31 and vWF expressions were increased and immunofluorescence results were stronger in the heme+HPX group.As compared with that in the control group ([41.0±7.18]/field),the number of tube 1 ike structure formation in the heme group ([25.0±3.67] /field) was significantly smaller (P＜0.05);as compared with that in the heme group,the number of tube like structure formation in the heme+HPX group ([58.6±2.70]/field) was significantly larger (P＜0.05).Conclusion HPX shows significant effect on promoting EPCs differentiation and new blood vessels formation to protect the endothelial integrity from toxic heme by increasing the HO-1 expression.
