Role of dexmedetomidine in lipopolysaccharide-induced macrophage inflammatory response and nuclear transcription factor-κB signal pathway
10.3760/cma.j.issn.1671-8925.2017.06.008
- VernacularTitle:右美托咪啶减轻LPS诱导的巨噬细胞炎症反应及其对NF-κB信号通路的影响
- Author:
Nianliang ZHANG
1
;
Xianglan LIU
;
Bo SUN
;
Haihua SHAN
Author Information
1. 济宁医学院附属日照市人民医院麻醉科
- Keywords:
Dexmedetomidine;
Sepsis;
Inflammatory cytokine
- From:
Chinese Journal of Neuromedicine
2017;16(6):581-584
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of dexmedetomidine (DEX) in lipopolysaccharide (LPS)-induced release of cell cytokines from monocyte macrophage RAW264.7 and nuclear transcription factor (NF)-κB signal pathway. Methods RAW264.7 cell lines were seeded in 6-well plates with a density of 1×106/mL (2 mL/hole) and randomly divided into control group (PBS), DEX group (10 ng/mL), LPS group (1 μg/mL) and LPS (1 μg/mL)+DEX (10 ng/mL) group. After incubation of 3, 6, 12 and 24 h, ELISA was employed to detect the concentrations of tumor necrosis factor (TNF)-α, interleukin (IL)-1βand high mobility group box (HMGB)-1 in the supernatants of 6 wells in each group. Then, 6 wells in each group were chosen at incubation of 12 h for determination of NF-κB signal pathway related proteins phosphorylated (p)-P65 and p-P50 expressions by Western blotting. Results As compared with those in the control group, the concentrations of TNF-α, IL-1β and HMGB-1 in the supernatant of LPS group were significantly increased (P<0.05), and the expressions of p-P65 and p-P50 were significantly increased in the LPS group (P<0.05). As compared with those in the LPS group, the levels of TNF-α, IL-1β and HMGB-1 were statistically decreased, and the expressions of P65 and P50 were significantly down-regulated in the DEX group (P<0.05). Conclusion DEX could inhibit the release of cells cytokines from RAW264.7 and decrease the levels of NF-κB signal pathway related protein expressions.
