Effect of SNORD47 over -expression on proliferation and invasion of U87 -epidermal growth factor receptorvⅢ glioma cells
10.3760/cma.j.issn.1671-8925.2017.06.002
- VernacularTitle:过表达SNORD47对U87-EGFRvⅢ胶质瘤细胞增殖和侵袭的影响
- Author:
Bin XU
1
;
Minhua YE
;
Shigang LYU
;
Miaojing WU
;
Bing XIAO
;
Yanghua FAN
;
Yi CHAI
;
Xingen ZHU
Author Information
1. 南昌大学第二附属医院神经外科
- Keywords:
SNORD47;
Glioma;
Proliferation;
Invasion;
Migration;
Matrix metalloproteinase
- From:
Chinese Journal of Neuromedicine
2017;16(6):547-552
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of SNORD47 over-expression on proliferation and invasion of U87-epidermal growth factor receptor (EGFR)vⅢ glioma cells. Methods U87-EGFRvⅢ glioma cells at logarithmic phase were assigned into lenti-SNORD47 group, lenti-NC group and blank control group. The recombinant lentiviruses containing lenti-SNORD47 or lenti-NC were transfected into U87-EGFRvⅢ glioma cells of the lenti-SNORD47 group and lenti-NC group, respectively. Forty-eight h after transfection, the SNORD47 expression in the three groups was measured by real time quantitative PCR. Western blotting was used to detect the protein expressions of matrix metalloproteinase (MMP) 2 and MMP9. The proliferation of U87-EGFRvⅢ cells 4, 24, 48, 72 and 96 h after transfection was evaluated by CCK-8 assay and colony formation assay. Transwell assay and wound-healing assay were used to examine the invasion and migration of these cells. Results The SNORD47 expression in the lenti-SNORD47 group was significantly higher than that in the lenti-NC group and control group (P<0.05). The protein expressions of MMP2 and MMP9 in the lenti-SNORD47 group were significantly decreased as compared with those in the lenti-NC group and control group (P<0.05). At 48, 72 and 96 h after transfection, the optical density and number of cloned cells in the lenti-SNORD47 group were significantly decreased as with those in the lenti-NC group and control group (P<0.05). The invasion and migration abilities of U87-EGFRvIII cells in the lenti-SNORD47 group were significantly suppressed as compared with those in the lenti-NC group and control group (P<0.05). Conclusion SNORD47 could inhibit the proliferative and invasive abilities of U87-EGFRvⅢ glioma cells.
