Early diagnosis of Parkinson's disease by PET/CT apoptosis imaging with tracer of 18F-ML-10
10.3760/cma.j.issn.1671-8925.2017.05.004
- VernacularTitle:18F-ML-10PET/CT凋亡显像早期诊断帕金森病的实验研究
- Author:
Shiming HUANG
1
;
Wei CHEN
;
Jinming ZHANG
;
Liang YIN
;
Jianlan YUE
;
Yanfeng LI
;
Wenjie JI
;
Xin ZHOU
;
Yongqiang MA
;
Huipeng MENG
;
Zhichun LIN
Author Information
1. 天津武警后勤学院附属医院核医学科
- Keywords:
18F-ML-10;
PET/CT;
Parkinson's disease;
Dopaminergic neuron;
Apoptosis
- From:
Chinese Journal of Neuromedicine
2017;16(5):452-457
- CountryChina
- Language:Chinese
-
Abstract:
Objective The 18F-ML-10 PET/CT apoptosis imaging was taken in the early Parkinson's disease (PD) rat models.The feasibility of diagnosis of PD with 18F-ML-10 PET/CT apoptosis imaging is explored.Methods Twenty adult healthy male Sprague-Dawley rats were randomly divided into control group and PD model group (n=l 0).Intrastriat administration of 6-hydroxy dopamine (6-OHDA) was performed to induce progressive and retrograde degenerative changes in the substantial nigra of neurons (PD models).One week after apomorphine inducement,the rotational behaviors of the rats were evaluated.~C-CFT PET/CT imaging and 18F-ML-10 PET/CT were performed to observe the dopamine transport protein expression in the corpus striatum and apoptosis of dopaminergic neurons in the substantia nigra.Immunofluorescence staining of anti-tyrosine hydroxylase (TH) antibody was performed to evaluate the survival of dopaminergic cells in the compact part of substantia nigra.TUNEL was performed to evaluate the apoptosis of dopaminergic cells in the compact part of substantia nigra.Nissl staining was performed to detect the cellular morphology.Results One week after PD modeling,the rats in the experimental group obviously rotated to the contralateral,and the average rotation speed was (4.52 ±1.03) r/min.The ratio of 11C-CFT between the right and left striatum of rats in the experimental group was 0.556±0.017,which was significantly lower than that of the control group (0.998±0.013,P＜0.05).The radioactivity ratio of'8F-ML-10 between the right and left substantia nigra of rats was 1.722±0.083,which was significantly higher than that of the control group (1.024±0.056,P＜0.05).Immunofluorescence showed that the ratio of TH-positive neurons between the right and left compact part of substantia nigra in rats of the experimental group was 0.528 ±0.012,which was significantly lower than that of the control group (1.036±0.030,P＜0.05).TUNEL showed that the number of dopaminergic neuronal apoptosis in the experimental group was 43.200±2.507,which was significantly larger than that of the control group (1.400±0.427,P＜0.05).Conclusion PD is associated with apoptosis of dopaminergic neurons;18F-ML-10 PET/CT imaging can be used to diagnose PD in the early-stage.
