Fragmentation of Golgi apparatus and expression changes of Rab30 in neurons
10.3760/cma.j.issn.1671-8925.2017.04.009
- VernacularTitle:神经元高尔基体碎裂与Rab30表达变化的关系
- Author:
Jia WANG
1
,
2
;
Hongfu YUAN
;
Wensheng ZHOU
Author Information
1. 410016 长沙,湖南省人民医院湖南省老年医学研究所
2. 410016 长沙,湖南省人民医院卒中中心
- Keywords:
Golgi apparatus;
Rab30;
HT22 neuron;
Thapsigargin
- From:
Chinese Journal of Neuromedicine
2017;16(4):369-373
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relation between fragmentation of the Golgi apparatus and expression changes of Rab30 in HT22 neurons.Methods The routinely cultured HT22 neurons were divided into control group,thapsigargin 3 h group and thapsigargin 5 h group;neurons in the thapsigargin 3 h group and thapsigargin 5 h group were given 0.1 μg/mL thapsigargin treatment for 3 or 5 h,respectively.Morphology of Golgi apparatus and location of Rab30 were observed with cytochemistry immunofluorescence.The Rab30 mRNA expression was detected by real-time fluorescent quantitative PCR.The protein expressions ofRab30,cis-Golgi matrix protein 130 (GM130),and golgi anti-apoptotic protein (GAAP) were detected by Western blotting.Results Thapsigargin induced markedly Golgi fragmentation in HT22 neurons.The longer the neurons were exposed to thapsigargin,the more the Golgi fragmentation.Hippocampal neurons also expressed Rab30,which was mostly localized on Golgi apparatus.The Rab30 mRNA and protein expressions in the control group,thapsigargin 3 h group and thapsigargin 5 h group were decreased in sequence (relative transcript level:1.00±0.00,0.73±0.05 and 0.53±0.01),with significant differences (P＜0.05).The GM130 and GAAP protein expressions in control group,thapsigargin 3 h group and thapsigargin 5 h group were decreased in sequence,with significant difference (P＜0.05).Conclusion Thapsigargin can induce Golgi fragmentation,which may be related to the decreased expression of Rab30 in HT22 neurons.
