Effect of berberine chloride on autophagy and β-secretase level in mice with traumatic brain injury
10.3760/cma.j.issn.1671-8925.2017.03.007
- VernacularTitle:盐酸小檗碱对颅脑外伤模型小鼠自噬和β-分泌酶影响的初步研究
- Author:
Shuxuan HUANG
1
;
Feiqi ZHU
;
Jinhua ZHU
;
Yuan LIU
;
Chunchun CHEN
;
Weifeng LIN
;
Zhong PEI
Author Information
1. 汕头大学医学院附属粤北人民医院神经内科
- Keywords:
Traumatic brain injury;
Berberine chloride;
Autophagy;
Beta-secretase
- From:
Chinese Journal of Neuromedicine
2017;16(3):251-255
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of berberine chloride on autophagy and β-secretase (BACE) level in mice with traumatic brain injury (TBI).Methods Eighteen female healthy C57BL/6 mice were randomly divided into three groups:control group,model group and berberine group (n=6).TBI models in the later two groups were established by a weight-drop hitting device and mice in berberine group were administered intragastricly with berberine chloride (50 mg/kg) once daily for 21 d.Immunofluorescent staining were used to assess LC3 and BACE expressions in ipsilateral cortex or thalamus,and then,their mean fluorescence intensities were calculated and compared among these three groups.Results LC3 expression in the ipsilateral cortex and thalamus and BACE expression in the ipsilateral cortex (0.02±0.01,0.06±0.02 and 0.04±0.01 in the control group,model group and berberine group) showed significant difference among the three groups (P＜0.05):LC3 expression in ipsilateral cortex and thalamus and BACE expression in the ipsilateral cortex of the model group were significantly increased as compared with those of the control group (P＜0.05);the LC3 expression in the ipsilateral cortex and thalamus and BACE expression in the ipsilateral cortex of the model group were significantly decreased as compared with those of the berberine group (P＜0.05).Conclusion Autophagy is over-activated in the ipsolateral cortex and thalamus and BACE is over-activated in the ipsolateral cortex after TBI,and these changes are significantly suppressed by berberine chloride.
