Changes of microglia phenotypes in rat models following repetitive mild traumatic brain injury
10.3760/cma.j.issn.1671-8925.2017.03.006
- VernacularTitle:小胶质细胞表型在反复轻度脑创伤模型大鼠中的变化研究
- Author:
Ruojing BAI
1
;
Huabin GAO
;
Zhaoli HAN
;
Shan HUANG
;
Xintong GE
;
Fanglian CHEN
;
Ping LEI
Author Information
1. 天津医科大学总医院保健医疗部(老年病科)、天津市老年病学研究所
- Keywords:
Repetitive mild traumatic brain injury;
Microglia subset;
M1 type;
M2 type
- From:
Chinese Journal of Neuromedicine
2017;16(3):246-250
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of microglia cell phenotypes in the injured brains of rats following repetitive mild traumatic brain injury (rmTBI).Methods Sixty male SD rats were randomly divided into sham-operated group,injury of one-week group,injury of two-week group,injury of four-week group,and injury of six-week group (n=12).Rats in the injury groups were induced rmTBI models by controlled cortical impact (CCI),and rats in the sham-operated group were only performed bone window opening without hitting.Six rats from each group were sacrificed;immunofluorescent staining was used to detect the Iba-1 positive microglial cells in the injured cortex and changes ofmicroglia subsets in the injured brain after rmTBI were analyzed by flow cytometry.Results As compared with the sham-operated group,the injury of one-week group and injury of six-week group had significantly increased percentages of Iba-1 positive microglial cells in the injured cortex (19％ and 12％,P＜0.05).flow cytometry indicated that CD451ow/CD11b+ cells were the microglial cells,accountting for 90％ ofCD11b+ cells;as compared with the sham-operated group,the injury of one-week group,injury of two-week group,injury of four-week group,and injury of six-week group had significantly increased M1 type microglial cells (P＜0.06),with injury of six-week group enjoying the highest level;as compared with the sham-operated group,the injury of one-week group,injury of two-week group and injury of four-week group had significantly increased M2 type microglial cells (P＜0.06),with injury of two-week group enjoying the highest level.Conclusion Dynamic changes ofmicroglia subsets after rmTBI are noted,which reveals that different subsets of microglia phenotypes might play their unique roles in the acute or chronic phases after rmTBI.
