Neuroprotective effect of 2-methoxyestradiol on neurological function and nerve cell apoptosis in rats with acute cerebral infarction
10.3760/cma.j.issn.1671-8925.2017.02.006
- VernacularTitle:2-甲氧基雌二醇对脑梗死大鼠神经功能及神经细胞凋亡的影响
- Author:
Jinghua PENG
1
;
Fei JIN
;
Hongri ZHANG
;
Yude ZHANG
Author Information
1. 河南科技大学第一附属医院神经内科
- Keywords:
2-methoxyestradiol;
Cerebral infarction;
Apoptosis;
Neurological function
- From:
Chinese Journal of Neuromedicine
2017;16(2):139-142
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the neuroprotective effect of 2-methoxyestradiol (2ME2) on neurological functions and nerve cell apoptosis in rats with acute cerebral infarction.Methods Seventy-two adult male SD rats were randomly divided into sham-operated group,model group and treatment group (n=24).Middle cerebral artery infarction models in the later two groups were constructed by Longa method.Rats in treatment group were treated with 2ME2 (24 mg/kg) daily for 7 d,while rats in the sham-operated group and model group with physiological saline.Bederson standard scale was adopted to analyze the nerve function defect 7 d after operation.Caspase-3 protein expression in the rat brain tissues was analyzed by Western blotting and apoptosis index was analyzed by TUNEL.Results As compared with those in the model group (2.015±0.871),Bederson standard scale scores in the treatment group (1.235±0.849) were significantly lower (P＜0.05);as compared with the sham-operated group (0.286±0.160,7.8 ±6.9),the model group and treatment group had significantly increased Caspase-3 protein level and apoptosis index (P＜0.05),while Caspase-3 protein level and apoptosis index in treatment group (0.615±0.185,27.8±6.4) were significantlylower than those in the model group (1.109±0.224,34.9±5.9,P＜0.05).Conclusion 2ME2 can improve the neurological function,reduce the level of apoptosis in rat brain nerve cells and has a protective effect on nerve.
