Effect of environmental enrichment on expressions of phosphorylated cAMP response element binding protein and apoptotic regulated genes in the hippocampus of immature rats with hypoxic-ischemic brain damage
10.3760/cma.j.issn.1671-8925.2017.02.005
- VernacularTitle:丰富环境对缺氧缺血性脑损伤幼鼠海马区pCREB及凋亡调控基因表达的影响
- Author:
Aiyun YUAN
1
;
Mei HOU
;
Ruobing SHAN
;
Qiuyan LIU
;
Yangyang GONG
;
Xiangfeng SUN
Author Information
1. 266034,青岛市妇女儿童医院神经康复科
- Keywords:
Hypoxic-ischemic brain damage;
Environmental enrichment;
cAMP response element binding protein;
Bcl-2;
Bax
- From:
Chinese Journal of Neuromedicine
2017;16(2):133-138
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of environmental enrichment intervention on spatial memory function,expressions of phosphorylated cAMP response element binding protein (pCREB) and apoptotic regulated genes (Bcl-2 and Bax) in the hippocampus of immature rats with hypoxic-ischemic brain damage (HIBD).Methods Two kinds of raising environments were chosen:standard environment and environmental enrichment.Thirty-two Wistar rats aged 7 days were randomly divided into sham-operated group (n=10),environmental enrichment group (n=11) and standard environment group (n=11).Rats in both environmental enrichment group and standard environment group were induced animal models of HIBD using the way of Rice-Vannucci.Rats in the sham-operated group and standard environment group were raised in standard environment,while rats in the environmental enrichment group were raised in environmental enrichment.After 21 days of environmental enrichment intervention,the escape latency for searching the hidden platform of immature rats was detected by Morris water maze navigation test.The pCREB protein expression was detected by Western blotting;Bcl-2 and Bax expressions in the hippocampus were detected by immunohistochemisty.Results (1) On the fourth and fifth days of Morris water-maze test,the escape latency in the environmental enrichment group was shorter significantly as compared with that in the standard environment group (P＜0.05).(2) As compared with the lesion lateral hippocampus of standard environment group,the expressions ofpCREB (0.435±0.121 vs.0.756±0.101) and Bcl-2 (0.103±0.028 vs.0.165±0.017) in the lesion lateral hippocampus of environmental enrichment group were significantly higher (P＜0.05);however,the expression of Bax in the environmental enrichment group was statistically lower (0.402± 0.028 vs.0.325±0.019,P＜0.05).Conclusion Environmental enrichment intervention can improve the spatial memory ability in the hippocampus after HIBD,which indicates that the effectiveness of environmental enrichment intervention might be through the pathway of CREB-pCREB-apoptotic regulated genes (Bcl-2 and Bax).
