Expressions of brain derived neurotrophic factor and tropomyosin-related kinase B and their significances in rats with early brain injury after subarachnoid hemorrhage
10.3760/cma.j.issn.1671-8925.2016.11.009
- VernacularTitle:BDNF和TrkB在大鼠蛛网膜下腔出血后早期脑损伤中的表达及意义
- Author:
Hongliang GE
1
;
Yuanxiang LIN
;
Zhangya LIN
;
Lianghong YU
;
Yuanlong ZHANG
;
Dezhi KANG
;
Chenyu DING
Author Information
1. 福建医科大学附属第一医院神经外科
- Keywords:
Subarachnoid hemorrhage;
Early brain injury;
Brain-derived neurotrophic factor;
Tropomyosin-related kinase B
- From:
Chinese Journal of Neuromedicine
2016;15(11):1130-1135
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expressions of brain derived neurotrophic factor (BDNF) and tropomyosin-related kinase B (TrkB) in rats with early brain injury (EBI) after subarachnoid hemorrhage,and study the neuroprotective effects of BDNF and TrkB on EBI.Methods Male Sprague-Dawley rats (n=56),weighing 280-320 g,were randomly divided into sham-operated group and SAH group;SAH models were established by endovascular perforation ofinternal carotid artery.At 24 and 72 h after modeling,neurological scale scores were recorded;brain water content was measured;immunohistochemical staining and ELISA were used to observe the dynamical expressions of BDNF and TrkB in the brain.Results At 24 and 72 h after modeling,the neurological function scores and brain water content of SAH rats were higher than those of sham-operated group.The expression scores of BDNF in the SAH rats were 1.33±0.52 and 1.67±0.52,and the expression levels were (12.11±0.44) mg/mL and (15.82±0.89) mg/mL;the expression scores of TrkB were 1.17±0.75 and 2.00±0.00,and the expression levels were (18.89±0.38) mg/mL and (25.18±0.68) mg/mL.The expression scores of BDNF in the sham-operated group were 0.33±0.52 and 0.17±0.41,and the expression levels of BDNF in the sham-operated group was (4.92±0.16) mg/mL and (4.93±0.20) mg/mL;the expression scores of TrkB were 0.17±0.41 and 0.33±0.52,and the expression levels were (8.52±0.41) mg/mL and (8.08±0.34) mg/mL.There were significant differences in BDNF and TrkB expressions between the two groups at 24 and 72 h after modeling (P＜0.05).Conclusion The expressions of BDNF and TrkB increase significantly after SAH,and BDNF and TrkB play protective effect on EBI after SAH.
