Effect of exogenous hydrogen sulfide on depression rat models caused by chronic stress
10.3760/cma.j.issn.1671-8925.2016.10.005
- VernacularTitle:外源性硫化氢对慢性应激所致抑郁大鼠模型的影响
- Author:
Chang PENG
1
;
Jingjing LUO
;
Mingchen XIAO
;
Xiaomei LUO
Author Information
1. 遵义医学院附属医院儿科
- Keywords:
Hydrogen sulfide;
Depression;
Stress;
Mineralocorticoid receptor;
Glucocorticoid receptor
- From:
Chinese Journal of Neuromedicine
2016;15(10):997-1003
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of exogenous hydrogen sulfide (H2S) donor NaHS on depression rat models induced by chronic stress,and provide a new idea for preventing and treating depression.Methods Thirty Sprague Dawley rats were randomly divided into normal control group,model group,model+NarHS group,model+normal saline group and normal control+NaHS group (n=6).Depression models were induced by sustained stimulations for three weeks;rats in the model+NaHS group and model+normal saline group were performed intraperitoneal injection of NaHS (10 mg/kg) or equivalent normal saline once a week,respectively.The weight,food intake,water intake,sugar water preference,adrenal gland weight index and behavioral indexes were observed in these rats;the hippocampus of the rats were collected,and the mRNA and protein expressions of mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in the hippocampus of rats were assayed by real-time PCR and Western blotting,respectively;the concentrations ofinterleukin (IL)-6 and tumor necrosis factor (TNF)-cα in the serum were assayed by ELISA,and hippocampal neurons were observed by Nissl staining.Results The weight,food intake,water intake,sugar water preference,number of horizontal movement and vertical erect of rats in the model+NaHS group were significantly decreased as compared with those in model group (P＜0.05),while opposite trend was noted in the adrenal index,with significant difference (P＜0.05).The contents of malonaldehyde,corticosterone,adrenocorticotropic hormone,IL-6 and TNF-α in the model group were significantly increased as compared with those in the normal control group (P＜0.05),but the indexes mentioned above,except for TNF-α,in the model+NaHS group were attenuated significantly as compared with those in the model group (P＜0.05).The results of real-time PCR and Westem blotting showed that the mRNA and protein expressions of GR and MR in hippocampal tissues of rats in the model group were statistically decreased as compared with those in the normal control group (P＜0.05),but the mRNA and protein expressions of GR and MR in hippocampal tissues of rats in model+NaHS group were significantly higher than those in the model group (P＜0.05).Nissl staining showed decreased level of nissl bodies and disordered arrangement of pyramidal cells in the hippocampus of the model group;however,model+NaHS group had alleviated loss ofnissl bodies and disordered arrangement of pyramidal cells in CA3 area of hippocampus as compared with the model group.Conclusion Chronic stress could lead to depression of rats,and exogenous H2S donor NarHS could attenuate chronic stress induced-depression of rats by up-regulating the expressions of GR and MR and down-regulating the content of stress hormone in rats.
