Effects of electroacupuncture on hippocampal ERK/CREB expressions and behaviors in depression model rats
10.3760/cma.j.issn.1671-8925.2016.09.008
- VernacularTitle:电针对抑郁症大鼠模型行为学及海马ERK/CREB信号通路表达的影响
- Author:
Rui CHEN
1
;
Yanling SHE
;
Ting JIANG
;
Shifen XU
;
Cheng LI
Author Information
1. 广东省第二人民医院广东省传统医学与运动伤害康复研究所
- Keywords:
Depression;
Electroacupuncture;
Extracellular signal-regulated kinase;
cAMP-response element binding protein
- From:
Chinese Journal of Neuromedicine
2016;15(9):913-917
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effects of electroacupuncture (EA) on behaviors and hippocampal extracellular regulated protein kinases (ERK)/cAMP-response element binding protein (CREB) expressions in depression model rats.Methods Forty male SD rats were divided into 4 groups randomly:normal group,model group,EA group and sham EA group (n=10).Chronic unpredictable mild stress (CUMS) and isolation were performed to establish the depression models.EA was applied to Yintang (EX-HN3) and Baihui (GV20) 10 min once a day for two weeks in EA group;sham EA group received placebo treatment.After 4 weeks of intervention,the sucrose intake test,forced swim test and Morris water maze test were used to measure the antidepressant-like effect of EA treatment.The expressions of ERK and CREB in the hippocampus were detected by Western blotting.Results As compared with the model group,the EA group had significantly improved sucrose solution intake ([62.080±11.995] ％ vs.[74.881±3.308] ％) and statistically decreased immobility time ([123.668± 18.925] s vs.[93.012±15.957] s) in forced swimming (P＜0.05).In Morris water maze test,the escape latency to find the target platform became gradually shorter and the frequency of crossing platform significantly increased in EA group (0.900±1.875 vs.3.200±1.549,P＜0.05).The phosphorylated(p)-ERK and p-CREB levels in the EA group were significantly increased as compared with those in the model group (P＜0.05).Conclusion EA could improve depression-like behaviors by increasing the levels of p-ERK and p-CREB in the hippocampus.
