Expression of miR-126 in brain ischemic injury tissues of diabetic rats and its significance in angiogenesis
10.3760/cma.j.issn.1671-8925.2016.09.007
- VernacularTitle:miR-126在糖尿病大鼠脑缺血损伤组织中的表达及在血管再生中的意义
- Author:
Xiaoyun LI
1
;
Lixin WANG
;
Jianyi NIU
;
Hongbing CHEN
Author Information
1. 潍坊医学院附属益都中心医院神经内科
- Keywords:
Micro RNA-126;
Diabetes mellitus;
Cerebral ischemic injury;
Angiogenesis
- From:
Chinese Journal of Neuromedicine
2016;15(9):908-912
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of micro RNA (miR)-126 in brain ischemic injury tissues of diabetic rats and its significance in angiogenesis.Methods Fifty adult female rats were randomly divided into five groups according to random number table:diabetic group,cerebral ischemia group,diabetic+cerebral ischemia group,miR-negative+diabetic+cerebral ischemia group and miR-126 mimics+diabetic+cerebral ischemia group (n=10).The models of diabetic and (or) focal cerebral ischemia were esblished,respectively.The rats in the later two groups were injected with 10 μg miR-negative control or 10 μg miR-126 mimics through ateral ventricle.Twenty-four h after cerebral ischemia modeling,the neurological functions of rats were assessed by using Zea-Longa scoring criteria,and infarct areas in the brain slices were analyzed by TTC staining.The expressions of miR-126,platelet-endothelial cell adhesion molecule-1 (PECAM-1),vascular endothelial cadherin (VE-cad) and vascular endothelial growth factor (VEGF) in the ischemic cortex tissues were detected by real time-PCR.Results The neurological scale scores and cerebral infarct volumes of rats in miR-negative+diabetic+cerebral ischemia group and diabetic+cerebral ischemia group were significantly higher than those in the diabetic group and cerebral ischemia group (P＜0.05);and those in the miR-126 mimics+diabetic+cerebral ischemia group were significantly lower than those in the miR-negative+diabetic+cerebral ischemia group,diabetic cerebral ischemia group and cerebral ischemia group (P＜0.05).The relative mRNA expression levels of miR-126,PECA M-1,VE-cad and VEGF in the ischemic cortex tissues of cerebral ischemia group,diabetic+cerebral ischemia group,miR-negative+diabetic+cerebral ischemia group were significantly lower than those in the diabetic group (P＜0.05);those in the diabetic+cerebral ischemia group and miR-negative+diabetic+cerebral ischemia group were significantly lower than those in the cerebral ischemia group (P＜0.05);those in the miR-126 mimics+diabetic cerebral ischemia group were statistically higher than those in the diabetic group,cerebral ischemia group,diabetic cerebral ischemia group and miR-negative+diabetes cerebral ischemia group (P＜0.05).Conclusion Up-regulation of miR-126 could help relieve diabetic ischemic brain tissue injury and improve neurological function,which might be related to the regulation ofangiogenesis after cerebral ischemic injury.
