Effect of docosahexaenoic acid on long-term learning and memory disorders after hypoxic ischemic brain damage in rats
10.3760/cma.j.issn.1671-8925.2016.07.007
- VernacularTitle:DHA对新生大鼠缺氧缺血性脑损伤后远期学习记忆障碍的影响
- Author:
Cheng ZENG
1
;
Siyun SHU
;
Yusha HUANG
;
Yan CHENG
;
Jun CHEN
;
Bin WANG
Author Information
1. 南方医科大学珠江医院儿科中心
- Keywords:
Docosahexaenoic acid;
Hypoxic-ischemic brain damage;
Learning and momery disorder;
Hypocampus;
Marginal division of striatum;
N-Methyl-D-aspartic acid receptor 1
- From:
Chinese Journal of Neuromedicine
2016;15(7):678-684
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of docosahexaenoic acid (DHA) on long-term learning and memory disorders and potential mechanism in rats after hypoxic ischemic brain damage. Methods Sixty neonatal 7-day-old SD rats were ramdonly divided into three groups: group S (sham operation+vehicle treatment), group C (hypoxic-ischemic brain damage [HIBD]+vehicle treatment) and group D (HIBD+DHA treatment). After left common carotid artery was isolated and ligated for 2.5 h, rats of group C and group D were put into a condition which oxygen concentration was about 8%for 2 h;rats in the group S were only isolated the left carotid artery, without ligation or hypoxia treatment;rats in the group D were intraperitoneally injected DHA of 15 mg/kg after modeling, and rats in the group S and group C were intraperitoneally injected equivalent volume of vehcle, once a day for 10 consecutive days. The pathomorphology changes of the hypocampal CA1 area, and marginal division of striatum were observed by Nissl staining 48 h after modling; the apoptosis cells were measured by TUNEL;immunohistochemical method was used to detect the expressions of Bax and Caspase-3 positive cells in the two brain areas. Morris water maza test was used to evaluate the long-term lerning and momory functions of 2-month-old rats, and the expressions of N-methyl-D-aspartate receptor 1 (NMDAR1) positive cells were detected by immunohistochemical method. Results The pathomorphology damage was significantly improved, the expressions of Bax and Caspase-3 positive cells and the neuron apoptosis in hypocampal CA1 areas and marginal division of striatum in group D were all signficantly decreased as compared with those in the group C (P<0.05). Rats in group D had significantly decreased escape latency as compared with those in group C in Morris water maze test (P<0.05), and the expression of NMDAR1 positive cells in the two brain areas of group D was significantly increased as compared with that in the group C (P<0.05). Conclusion DHA has the ameliorative effect on long-term learning and memory disorders after hypoxic ischemic brain damage in rats, which may be associated with inhibitory action of cell apoptosis at early phase and up-regulation of expression of NMDA1 at the late phase.
