Animal models of cortical development malformation induced by methylazoxymethanol acetate
10.3760/cma.j.issn.1671-8925.2016.04.005
- VernacularTitle:甲基氧化偶氮甲醇诱导皮质发育障碍动物模型的研究
- Author:
Bing CHEN
1
;
Xiaolin YANG
;
Lihong LIU
;
Xin CHEN
;
Hui YANG
;
Shiyong LIU
Author Information
1. 第三军医大学新桥医院神经外科
- Keywords:
Malformation of cortical development;
Methylazoxymethanol acetate;
Rat model;
Epilepsy
- From:
Chinese Journal of Neuromedicine
2016;15(4):350-354
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the new animal models of malformation of cortical development (MCD) induced by methylazoxymethanol acetate (MAM),and analyze the relationship between extent of histopathology and age,seizure susceptibility and effect of seizure on the histopathology.Methods Pregnant Sprague-Dawley rats were randomly divided into MAM group and control group;rats in the MAM group received twice intraperitoneal injection of MAM (15 mg/kg) to establish MCD models on embryonic day 15,while rats in the control group only given normal saline.Daily activities and EEG features of the next generation rats were observed;Thioine-Giemsa staining and immunofluorescence staining of NeuN were applied to observe the histological changes.Pilocarpine was administrated to investigate the effects of seizure susceptibility and epilepsy seizure on pathological morphology.Results The MAM rats were dull and curtail their activities,their seizure susceptibility elevated.The histopathological and immunofluorescence staining results revealed that the MAM rats' cerebral cortex decreased and the laminar organization was disrupted;there were heterotopic neuros,hypertrophic neurons and proliferation nodules in the hippocampal and cortex;the pathology changes exacerbated with age.The malformation of cortical development was more serious in the MAM rats which experienced seizure than the MAM rats.Conclusion The MCD rat models can be established by twice intraperitoneal injection of MAM,and their pathological features resemble the patient of MCD and their pathology exacerbate with age.Seizure can aggravate the pathology of MCD.Combining utilization MAM and pilocarine could be a good approach to establish animal models of epilepsy based on MCD.
