Extremely low-frequency electromagnetic fields promote neuronal differentiation of rat mesenchymal stem cells via ERK signaling pathways
10.3760/cma.j.issn.1671-8925.2016.02.001
- VernacularTitle:超低频磁场基于ERK信号通路介导促进骨髓间充质干细胞向神经元样细胞分化
- Author:
Guangyong LIN
1
;
Haishan SHI
;
Wei LIU
;
Wen WU
Author Information
1. 南方医科大学珠江医院康复理疗科
- Keywords:
Extremely low frequency-electromagnetic field;
Bone marrow mesenchymal stem cell;
Neuron-like cell;
Neuronal differentiation;
ERK signaling pathway
- From:
Chinese Journal of Neuromedicine
2016;15(2):109-116
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of extremely low frequency-electromagnetic field (ELF-EMF) on bone mesenchymal stem cells (BMSCs) differentiating into neuron like cells in vitro and research its mechanism.Methods BMSCs were collected from rats by means of whole bone marrow adherent.Flow cytometry was used to assay cell surface marker at passage 3.And then,BMSCs were assigned into four groups:ELF-EMF group,ELF-EMF+U0126 (inhibitor) group,U0126 group and control group;cells were induced by medium (2％ DMSO and 200 μmol/L BHA) for 5 h.In the process of neural induction,ELF-EMF group and ELF-EMF+U0126 group were received 10 Hz,500 GS ELF-EMF stimulation.Besides,ELF-EMF+U0126 group and U0126 group were pretreated with 50 μmol/L extracellular signal-regulated kinase (ERK)1/2 inhibitor U0126.The morphology of BMSCs was observed under inverted microscope.The expression of nestin was detected by immunofluorescent staining and Western blotting to identify and determine the differentiation.Western blotting was applied to detect the preotein level of phosphorylase-ERK1/2 after ELF-EMF exposure.Results BMSCs presented a single long spindle morphology,growing with close whirlpoor-like arrangement;CD90 expression rate was up to 97.9％,while that of CD45 only 4.7％.After induction,each group of cells showed similar shape with neuron-like cells gradually.As compared with the other three groups,ELF-EMF group had significantly higher expression levels of nestin and phosphorylatd ERK1/2 detected by immunofluorescent staining and Westem blotting,respectively (P＜0.05).Meanwhile,the expression levels of nestin among the ELF-EMF+U0126 group,U0126 group and control group were not statistically significant (P＞0.05).Conclusion ELF-EMF could promote neuronal differentiation of mesenchymal stem cells via activation of ERK1/2 signaling pathways.
