Effect of brain-derived neurotrophic factor on apoptotic early events in hippocampal cells after status convulsivus
10.3760/cma.j.issn.1671-8925.2016.01.007
- VernacularTitle:脑源性神经营养因子对持续惊厥后海马细胞凋亡早期事件的影响
- Author:
Aiyun YUAN
1
;
Dianrong SUN
;
Xiaodan LIU
;
Mei HOU
Author Information
1. 青岛大学医学院附属妇女儿童医院神经康复科
- Keywords:
Status convulsivus;
Mitochondrial membrane potential;
Cytochrome C;
Brain-derived neurotrophic factor
- From:
Chinese Journal of Neuromedicine
2016;15(1):30-34
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of brain-derived neurotrophic factor (BDNF) on brain damage and its mechanism by investigating the influence of exogenous BDNF on mitochondrial membrane potential (△ψm) and cytochrome C (cytC) release in the hippocampus after status convulsivus (SC).Methods Sixty-four healthy adult Wistar rats were randomly divided into normal group (n=32) and SC group (n=32) which were injected with lithium-pilocarpine intraperitoneally for SC continuing 30 min.The rats in these two groups were intraventricularly injected (Ⅳ) with normal sodium (NS) 2 μL,BDNF 4 μL (0.1 μg/μL),or BDNF antibody 2 μL (200 μg/mL) in left lateral ventricle (or no injection),and sacrificed 6 h later.The levels of △ψm and cytC ofhippocampal cells were determined by flow cytometry.Results (1) As compared with subgroups of normal group,the levels of △ψm significantly decreased and cytC significantly increased in the SC subgroups,respectively (P＜0.05).(2) In normal group,the levels of △ ψm significantly decreased and cytC significantly increased in the lateral hippocampus of normal-anti-BDNF subgroup as compared with those in normal-non-Ⅳ subgroup (P＜ 0.05),and the levels of △ψm was significantly higher and cytC significantly lower in the lateral of normal-BDNF subgroup than those in the lateral ofnormal-anti-BDNF subgroup (P＜0.05).(3) In SC group,as compared with those in the lateral of SC-NS subgroup and SC-non-Ⅳ subgroup,the level of △ψm significantly decreased and cytC level significantly increased in the lateral of SC-anti-BDNF subgroup (P＜0.05),however they were reversed in the lateral of SC-BDNF subgroup (P＜0.05).Conclusions SC could induce △ψm decrease and cytC release ofhippocampal cells.The exogenous BDNF could partly inhibit the decrease of △ψm and the release of cytC in the lateral hippocampus after SC,which indicates that BDNF might protect the brain after SC through inhibiting the early event of apoptosis.
