Glioma stem cell differentiation induced by micro RNA 124 overexpression
10.3760/cma.j.issn.1671-8925.2015.12.002
- VernacularTitle:miR-124过表达诱导胶质瘤干细胞分化作用研究
- Author:
Zongping WU
1
;
Yang GUO
;
Qiming HUANG
;
Fabing ZHANG
;
Zhenzhou CHEN
Author Information
1. 351100,莆田市第一医院神经外科
- Keywords:
Micro RNA 124;
Glioma stem cell;
Inflammatory cytokine
- From:
Chinese Journal of Neuromedicine
2015;14(12):1196-1200
- CountryChina
- Language:Chinese
-
Abstract:
Objective Glioma stem cells (GSCs) are the root causes ofglioblastoma recurrence.Glioma expresses low level of micro RNA 124 (miR-124), which plays an important role in the development of glioma.In this study, we explore the mechanisms miR-124 by overexpressing miR-124 in GSCs.Methods Human GSCs were transfected with lentivirus mediated miR-124 overexpression vector (pGC-miR-124-GSCs group), and GSCs in normal culture were used as control group.Cell proliferation was accessed by MTT assay, and stem cell phenotype was analyzed by flow cytometry.Quantitative real time PCR was employed to detect the miR-124 expression and its target genes (A kt and RelA) expressions;and ELISA was carried out to detect the secretions of downstream inflammatory cytokines interleukin (IL)-1 and IL-8.Results The lentiviral miR-124 expression vector was constructed successfully and transfected into human GSCs (pGC-miR-124-GSCs).The proliferative capacity of cells in the pGC-miR-124-GSCs group was significantly lower than that in the control cells (P＜0.05).CD133 positive rate was statistically decreased, the miR-124 expression was significantly increased, the A kt and RelA expressions were significantly decreased, and correspondingly the secretions of IL-1and IL-8 were significantly reduced in the pGC-miR-124-GSCs group as compared with those in the control group (P＜0.05).Conclusion MiR-124 overexpression induces GSCs differentiation and activates a strong anti-tumor ability, whose mechanism may be related to inhibition of inflammatory cytokine production.
