Survival of retinal ganglion cells after intraocular transplantation of chitosan scaffold composited by ciliary neurotrophic factor transfected human umbilical cord blood stem cells in rats after optic nerve injury
10.3760/cma.j.issn.1671-8925.2015.11.014
- VernacularTitle:CNTF转染人脐血干细胞复合壳聚糖支架移植对视神经损伤大鼠视网膜神经节细胞存活的影响
- Author:
Baoguo LIU
1
;
Long ZHANG
;
Wei HUANG
;
Liming HE
;
Gang XIAO
Author Information
1. 粤北人民医院神经外科
- Keywords:
Ciliary neurotrophic factor;
Human umbilical cord blood stem cell;
Chitosan scaffold;
Optic nerve injury;
Retinal ganglion cell
- From:
Chinese Journal of Neuromedicine
2015;14(11):1152-1155
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the recovery effect of intraocular transplantation of chitosan scaffold composited by ciliary neurotrophic factor (CNTF) transfected human umbilical cord blood stem cells (hUCBCs) on intracranial optic nerve injury (IONI) in rats.Methods Eighty Wistar rats were randomly divided into five groups (n=16):normal control group,IONI model group,IONI+vehicle group,IONI+hUCBCs group and IONI+CNTF-hUCBCs-chitosan scaffold group;IONI models in the later four groups were established by microsurgery through subfrontal approach;and vitreous tissues in the rats of the later three groups were given saline injection,and hUCBCs and CNTF-hUCBCs-chitosan scaffold transplantation,respectively.Immunohistochemical method was employed to detect retinal ganglion cells (RGCs) expression and the number of the cells.Results As compared with the normal control group (1580.65±84.36),the IONI model group and IONI+vehicle group had significantly decreased number of RGCs (351.38±29.73 and 382.05±47.22,P＜0.05);as compared with the IONI model group and IONI+vehicle group,IONI+hUCBCs group and IONI+CNTF-hUCBCs-chitosan scaffold group had significantly increased number of RGCs (614.82±37.21 and 937.59±62.25,P＜0.05);and the difference between the IONI+hUCBCs group and IONI+CNTF-hUCBCs-chitosan scaffold group was significant (P＜0.05).Conclusion There is more significant repair effect of intraocular transplantation of chitosan scaffold composited by CNTF-transfected hUCBCs than pure hUCBCs transplantation on rats after IONI.
