Expression of Nav1.1 and its significance in brain tissues of FMR1 knockout mice
10.3760/cma.j.issn.1671-8925.2015.09.007
- VernacularTitle:FMR1基因敲除小鼠脑组织中Nav1.1的改变及其意义
- Author:
Wei DI
1
;
Hua LYU
;
Jun LIU
;
Weiping LIAO
;
Yonghong YI
Author Information
1. 陕西省人民医院神经内科
- Keywords:
Fragile X syndrome;
Navl.1;
Epilepsy;
Susceptibility
- From:
Chinese Journal of Neuromedicine
2015;14(9):894-898
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the possible role of Nav 1.1 in the pathogenesis of increased susceptibility to epileptic seizures in FMR1 knockout (FMR1 KO) mice.Methods FVB strain FMR1 KO mice and wild type (WT) controls at ages of 2 and 4 weeks old were chosen;immunohistochemistry was used to determine the expression of Nav 1.1 in different brain regions (striate cortex,temporal cortex,piriform cortex,hippocampus CA1,CA3 and dentate gyrus),and Western blotting was used to determine the Nav1.1 level in the cerebral acustici cortex and hippocampus.Results The mean optical density of Nav1.1 was significantly increased in the striate cortex,temporal acustici cortex,piriform cortex,regions of CA1 and dentate gyrus in FMR1 KO mice at ages of 2 and 4 weeks (2 weeks:0.058±0.006,0.054± 0.006,0.130±0.015,0.090±0.009 and 0.142±0.010;4 weeks:0.066±0.007,0.060±0.007,0.159±0.018,0.102±0.015 and 0.192±0.025) as compared with the age-matched WT mice (2 weeks:0.049±0.007,0.046±0.007,0.118±0.012,0.080±0.009 and 0.133±0.010;4 weeks:0.051±0.007,0.048±0.005,0.127± 0.012,0.089±0.012 and 0.175±0.024,P＜0.05).The levels of Nav1.1 in the cerebra1 cortex and hippocampus in FMR1 KO mice at ages of 2 and 4 weeks (2 weeks:0.635±0.082 and 0.954±0.111;4 weeks:0.819 ±0.064 and 1.145 ±0.159) were also significantly increased as compared with the age-matched WT mice (2 weeks:0.382±0.025 and 0.555±0.056;4 weeks:0.550±0.040 and 0.847±0.127,P＜0.05).Conclusion Increased expression of Nav1.1 might play an important role in the increased susceptibility to epileptic seizures in FMR1 KO mice.
