Changes of serum miR-145 and miR-497 levels and their correlations with high sensitive C-reactive protein and matrix metalloproteinase-9 in patients with ischemic stroke
10.3760/cma.j.issn.1671-8925.2015.08.003
- VernacularTitle:缺血性脑卒中患者血清中miR-145、miR-497表达变化及其与Hs-CRP、MMP-9的相关性
- Author:
Zhenzhao LUO
1
;
Man KONG
;
Zhongxin LU
;
Jing WANG
Author Information
1. 华中科技大学同济医学院附属武汉市中心医院检验科
- Keywords:
Ischemic stroke;
MiR-145;
MiR-497;
High sensitive C-reactive protein;
Matrix metalloproteinase-9
- From:
Chinese Journal of Neuromedicine
2015;14(8):770-774
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the changes of serum miR-145 and miR-497 levels and their correlations with high sensitive C-reactive protein (Hs-CRP) and matrix metalloproteinase-9 (MMP-9) in patients with ischemic stroke.Methods One hundred and twenty-six patients with ischemic stroke,including 28 mild neurologic impairment (NI) patients,61 moderate NI patients and 37 severe NI patients,and 107 normal controls,were collected in our hospital from January 2013 to October 2013.Expressions of serum miR-145 and miR-497 were measured by real-time fluorescent quantitative PCR,protein markers Hs-CRP and MMP-9 were determined by immune turbidimetry and ELISA assay,respectively,correlations between microRNAs (miRNAs) and protein markers were analyzied by partial correlation analysis.Results Both expressions of serum miR-145 and miR-497,and Hs-CRP and MMP-9 levels in ischemic stroke patients were significantly elevated as compared with those in the normal controls (P＜0.05).Positive correlations were noted between of serum miR-145 expression and both serum miR-497 expression and expressions of serum Hs-CRP and MMP-9 (r=0.718,P=0.000;r=0.658,P=0.000;r=0.455,P=0.000);meanwhile,serum miR-497 expression and expressions of serum Hs-CRP and MMP-9 were also positively correlated (r=0.845,P=0.000;r=0.787,P=0.000).Severe NI patients had significantly higher expressions of serum miR-145 and miR-497,and Hs-CRP and MMP-9 levels as compared with mild NI patients and moderate NI patients (P＜0.05),and these levels in the moderate NI patients were significantly higher than those in the mild NI patients (P＜ 0.05);these miRNAs and protein markers showed a different correlation strength depended on the different severities of neurologic impairment.Conclusion Both of serum miR-145 and miR-497 levels may be useful biomarkers for screening ofischemic stroke;and miR-145 and miR-497 may have synergistic effect on regulating the pathogenesis of ischemic stroke.
