Expressions of HMGB1 and TLR4 in human glioma tissues of patients combined with epilepsy
10.3760/cma.j.issn.1671-8925.2015.06.005
- VernacularTitle:伴有癫痫的人脑胶质瘤中HMGB1/TLR4的表达研究
- Author:
Shaolong ZHOU
1
;
Xinjun WANG
;
Xudong FU
;
Jianye WANG
;
Jixin SHOU
Author Information
1. 郑州大学第五附属医院神经外科
- Keywords:
Epilepsy;
Glioma;
HMGB1;
TLR4
- From:
Chinese Journal of Neuromedicine
2015;14(6):563-566
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of high mobility group protein b 1 (HMGPB1) and toll-like receptors 4 (TLR4) in human glioma tissues of patients with epilepsy and explore the mechanism of glioma-associated epilepsy.Methods Ninety-five glioma specimens,collected during the resection surgery in our hospital firom June 2011 to June 2014,were used in our experiment;among them,55 were low-grade gliomas and 40 were high-grade gliomas.Immunohistochemistry and real time-PCR were used to assay the protein and mRNA expressions of HMGB1 and TLR4 in glioma tissues and peritumoral tissues,and the relation between HMGB1/TLR4 and glioma-associated epilepsy was analyzed.Results The incidence of glioma-associated epilepsy was 35.79％ (34/95),and the incidence of glioma-associated epilepsy in low-grade gliomas (49.09％) was significantly higher than that in high-grade gliomas (17.50％)(x2=10.057,P=0.002).Immunohistochemisty and RT-PCR showed that the protein and mRNA expressions of HMGB1/TLR4 in the peritumoral tissues with glioma-associated epilepsy were significantly higher than those in the peritumoral tissues without glioma-associated epilepsy (P＜0.05),while no significant difference was noted between glioma tissues with and without epilepsy (P＞0.05).Conclusion Over-expression of HMGB1 and TLR4 in peritumoral tissues of glioma patients may be related with the development of glioma-associated epilepsy,which may be a new target in glioma therapy.
