Effect of sodium benzoate on learning and memory abilities and morphology of hippocampal neurons in rats
10.3760/cma.j.issn.1671-8925.2015.05.007
- VernacularTitle:安钠咖对大鼠学习记忆能力及海马神经元形态的影响
- Author:
Haixia LU
1
;
Lifang ZHANG
;
Jinshun QI
Author Information
1. 山西医科大学第一临床医学院
- Keywords:
Sodium benzoate;
Learning ability;
Memory ability;
Hippocampus;
Neuron
- From:
Chinese Journal of Neuromedicine
2015;14(5):464-468
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect ofintraperitoneal injection of sodium benzoate on learning and memory abilities and morphology of hippocampal neurons in rats.Methods Thirty-six male SD rats were randomly divided into three groups:control group,sodium benzoate low-dose group and sodium benzoate high-dose group (n=12);intraperitoneal injection of 1 mL normal saline,60 mg/kg sodium benzoate or 120 mg/kg sodium benzoate was performed on the rats of three groups,respectively,for 20 d.Morris water maze test (place navigation,space exploration and visible platform searching) was used to assess the spatial learning and memory of rats,and HE staining was used to observe morphological changes in hippocampal neurons.Results The escape latency of rats in the sodium benzoate high-dose group was statistically significant in the navigation test as compared with that in the control group and the sodium benzoate low-dose group (P＜0.05);no significant difference was noted between sodium benzoate low-dose group and control group (P＞0.05).In probe test,no significant differences in the number crossing target quadrant were found after withdrawal of platform between groups (P＞0.05).HE staining showed that hippocampal CA1 neurons were sparse,showing a disordered arrangement in sodium benzoate low-dose group;the hippocampal neurons of CA1 region and fascia dentata region in sodium benzoate high dose group showed messed arrangement,nuclear pyknosis and cytoplasmic concentration as compared with that in the control group.Conclusion The high-dose sodium benzoate can decrease the learning ability of rats and impair hippocampal neurons.
