Effect of high fat diet on stability of mitochondrial DNA/mRNA in the nerve cells of mice
10.3760/cma.j.issn.1671-8925.2015.03.007
- VernacularTitle:高脂饮食对小鼠神经细胞线粒体DNA/mRNA稳定性的影响
- Author:
Lang ZHANG
1
;
Dilijiang·Yiming A
;
Jie YIN
;
Liping GAO
;
Yuhong JING
Author Information
1. 兰州大学基础医学院人体解剖与组织胚胎学暨神经科学研究所
- Keywords:
Mitochondrial DNA/mRNA;
High fat diet;
Nerve cell
- From:
Chinese Journal of Neuromedicine
2015;14(3):249-253
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of high fat diet on stability of mitochondrial DNA/mRNA of frontal cortex tissue in mice.Methods Forty male mice were randomly divided into regular diet group and high fat diet group (n=20).All animals were sacrificed at 4 months (n=10) or 6 months (n=10) after experiment.The serum glucose,lipid and cholesterol levels were evaluated.And mitochondria were isolated in the frontal cortex of mice,and the changes ofmitochondrial DNA/mRNA were tested using PCR and real-time quantitative PCR.Results The serum glucose,lipid and cholesterol levels showed no significant changes in the mice of high fat diet group after feeding for 4 months as compared with those in the mice of regular diet group (P＞0.05),while the lipid level in the high fat diet group was increased significantly as compared with that in the regular diet group after feeding for 6 months (P＜0.05).The D-Loop level showed no significant changes after feeding for 4 and 6 months between the high fat diet group and the regular diet group (P＞0.05).Decreased ATPase6 and increasd Cyt3 and Cytb expression levels in the high fat diet group were noted as compared with those in the regular diet group after feeding for 6 months(P＜0.05).Conclusion The stability of mitochondrial DNA decreases in the brain of mice at the persistent feeding with high fat diet for 6 months; especially,pattem of mitochondrial mRNA expression changes obviously at early stage.
