Relation of peptidyl-prolyi isomerase 1 and Nanog expressions in human gliomas
10.3760/cma.j.issn.1671-8925.2015.03.003
- VernacularTitle:胶质瘤中PIN1-Nanog相关通路表达的研究
- Author:
Shengyuan NI
1
;
Chaoshi NIU
;
Yang YANG
;
Xiangyu ZHANG
Author Information
1. 230001合肥,安徽医科大学附属省立医院神经外科,脑功能与脑疾病安徽省重点实验室,安徽省脑立体定向神经外科研究所
- Keywords:
Glioma;
Peptidyl-prolyi isomerase 1;
Nanog
- From:
Chinese Journal of Neuromedicine
2015;14(3):227-232
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obsevre the peptidyl-prolyi isomerase 1 (PIN1) and Nanog expreesions in glioma tissues and the effect of PIN1 inhibitor polyiso-butylene (PiB) on their expressions and U87 cell proliferation.Methods Eighty-four glioma samples (15 with WHO graded Ⅱ,27 with WHO graded Ⅲ and 42 with WHO graded Ⅳ),collected in our hospital from March 2013 to August 2014,were chosen in our study; their expressions of PIN1 and Nanog were detected by immunohistochemical staining; U87 glioblastoma cell lines were cultured; 0.2,0.5,1.0 and 2.0 μg/mL PiB was given to the cells for 24 and 48 h,and then,MTT assay was employed to detect the inhibited effect and inhibition ratio was calculated; 1.0 μmol/L PiB group (U87 cells treated with 1.0 μmol/L PiB for 24 h) and control group (U87 cells without any treatment) were chosen,immunofluorescence staining was used to detect the PIN1 and Nanog protein expressions,and real time-PCR and Western blotting were employed to observe the mRNA and protein expressions of PIN1 and Nanog.Results The positive protein expression rate of PIN1 and Nanog in gliomas of different grades (WHO graded Ⅱ,Ⅲ and Ⅳ) was significantly different (P＜0.05):the higher the malignancy grade,the higher the rate of positive protein expression of PIN1 and Nanog.MTT assay indicated that PiB could inhibit the cell proliferation in dose-and concentration-dependent manners; as compared with the control group,1.0 μmol/L PiB group had significantly decreased percentages of PIN1+,Nanog+ and PIN1+/Nanog+ cells (P＜0.05); the mRNA PIN1 expression quantity in the control group and 1.0 μmol/L PiB group was 1.82±0.03 and 0.94±0.20,and that of Nanog was 1.47±0.04 and 0.82±0.19,with significant differences (P＜0.05); the protein PIN1 expression quantity in the control group and 1.0 μmol/L PiB group was 2.96±0.05 and 1.15±0.26,and that of Nanog was 1.52±0.16 and 0.75±0.05,with significant differences (P＜0.05).Conclusion PIN1 and Nanog play important roles in the pathogenesis of human gliomas and are related to the glioma malignancy; PIN1 may participate in the regulation of Nanog,and there is a pathway between the two genes; the glioma cell proliferation ability is suppressed by down-regulating the PIN1-Nanog expression.
