Effect of chronic treatment with levamisole on imidazoline I2 receptor in rats with experimental autoimmune encephalomyelitis
10.3760/cma.j.issn.1671-8925.2014.12.010
- VernacularTitle:左旋咪唑长期处理后对EAE大鼠咪唑啉2受体的影响
- Author:
Niange XIA
1
;
Jia LI
;
Yanyan CHEN
;
Weiyong YIN
;
Rongyuan ZHENG
Author Information
1. 温州医科大学附属第一医院神经内一科
- Keywords:
Levamisole;
Experimental autoimmune encephalomyelitis;
Imidazoline I2 receptor
- From:
Chinese Journal of Neuromedicine
2014;13(12):1228-1232
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of chronic treatment with levamisole (LMS) on imidazoline I2 receptor (I2R) in rats with experimental autoimmune encephalomyelitis (EAE).Methods Thirty female Wistar rats were randomly divided into three groups:control group (n=8),EAE model group (n=10),EAE+LMS treatment group (n=12); the rat models of EAE were induced by immunizing with guinea pigs spinal cord homogenate.Subcutaneous injection of 0.5 mL of normal saline+complete Freund's adjuvant (CFA) emulsion was performed to rats in the control group,and 0 and 24 h after the that,intraperitoneal injection of 0.4 mL of saline twice daily was performed; subcutaneous injection of 0.5 mL of homogenate+CFA emulsion was performed to rats in the EAE model group; subcutaneous injection of 0.5 mL of homogenate+CFA emulsion was performed to rats in the EAE+LMS treatment group,and 0 and 24 hafter the that,intraperitoneal injection of LMS (10 mg/kg) twice daily was performed.The severity of EAE was scored according to the signs and symptoms.Pathological changes were observed through hematoxylin-eosin staining and Luxol-Fast blue dyeing,and the degrees of inflammatory infiltration were evaluated.The maximal binding capacity (Bmax) and dissociation content (Kd) of I2R were measured by radioligand binding assay.Results As compared with rats in the EAE model group,rats in the EAE+LMS treatment group had lower incidence of EAE,alleviated clinical symptoms,prolonged latency and decreased central nervous system inflammation.Radioligand binding assay showed that both the Bmax values and Kd constant of I2R (266.1 ±28.13 fmol/mg and 5.307 ± 1.107) in the EAE model group were increased as compared with those in the control group (177.5±26.10 fmol/mg and 3.586±1.053,respectively),with statistically significant differences (P＜0.05).As compared with those in the EAE model group,the I2R Bmax values in the EAE+LMS treatment group (496.1±52.31 fmol/mg) were markedly increased (P＜0.05),but there were no significantly differences in Kd values of I2R between these two groups (P＞0.05).Conclusion Chronic treatment with LMS has beneficial neuroprotective effect on rats with EAE,and its mechanism might be related to the regulation of I2R.
