Effect of 2-methoxyestradiol on phosphorylation of Tau protein and motor function of rats after spinal cord injury
10.3760/cma.j.issn.1671-8925.2014.11.010
- VernacularTitle:2ME2对脊髓损伤大鼠运动功能及Tau蛋白磷酸化的影响
- Author:
Hongri ZHANG
1
;
Jinghua PENG
;
Maoyin ZHANG
;
Honglong ZHOU
;
Ruxiang XU
Author Information
1. 510280广州,南方医科大学珠江医院神经外科,广东神经外科研究所,广东省脑功能修复与再生重点实验室
- Keywords:
Spinal cord injury;
2-methoxyestradiol;
Tau protein;
Phosphorylation
- From:
Chinese Journal of Neuromedicine
2014;13(11):1127-1130
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 2-methoxyestradiol (2ME2) on phosphorylation of Tau protein (p-Tau) and mortor function of rats after spinal cord injury (SCI).Methods A total of 72 adult male Sprague-Dawley rats were randomly divided into 3 groups:sham-operated group (n=24),SCI group (n=24) and SCI+2ME2 treated group (n=24); models of SCI were created by modified Allen's method; one d after SCI,rats in the SCI+2ME2 treated group were given intraperitoneal injection of 2ME2 (24 mg/kg) for seven d,and rats in the SCI group were given the same volume of normal saline.Basso,Beatti,Bresnahan (BBB) scale was performed to evaluate the hindlimb function one,three,seven,14,21 and 28 d after SCI; the expression changes ofp-Tau (Ser 262) seven d after SCI were observed by immunofluorescence and Western blotting.Results BBB scale scores in the SCI group and SCI+2ME2 group were significantly increased following the prolonging of injury times (1-28 d of injury,P＜0.05); on the 7th,14th,21st and 28th d of injury,the scores in the SCI+2ME2 group were significantly higher than those in the SCI group (P＜0.05).The p-Tau positive cells in the spinal cord and the p-Tau (Ser 262) expression in the SCI group and SCI+2ME2 group were significantly increased as compared with those in the sham-operated group (P＜0.05),and those in the SCI+2ME2 group (19.05± 1.34 and 0.283±0.094) were obviously lower as compared with those in the SCI group (10.36±1.28 and 0.607±0.105,P＜0.05).Conclusion 2ME2 offers protection in rats with SCI by reducing the level of tau phosphorylation.
