Effect of 11 mHz ultra-low frequency transcranial magnetic stimulation on expressions of nestin and Brdu in the hippocampus of rats with focal cerebral ischemia and reperfusion
10.3760/cma.j.issn.1671-8925.2014.11.008
- VernacularTitle:11mHz超低频经颅磁刺激对局部脑缺血再灌注大鼠海马区巢蛋白、Brdu表达的影响
- Author:
Haishan SHI
1
;
Jianlan XU
;
Guangyong LIN
;
Wen WU
Author Information
1. 南方医科大学珠江医院康复理疗科
- Keywords:
Focal cerebral ischemia and reperfusion;
Transcranial magnetic stimulation;
Nestin
- From:
Chinese Journal of Neuromedicine
2014;13(11):1117-1122
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 11 mHz ultra-low frequency transcranial magnetic stimulation (ILF-TMS) on neural function recovery and expressions ofnestin and Brdu in the hippocampus of cerebral ischemia and reperfusion rats.Methods Seventy-two rats were used in our study and divided into 11 mHz ILF-TMS group,sham-stimulated group and sham-operated group (n=24);The filament method was used to establish the focal cerebral ischemia and reperfusion (I-R) rat models in the first two groups.Rats in the sham-operated group only performed blood vessel separation without ligation; rats in the 11 mHz ILF-TMS group were given magnetic stimulation at 11 rnHz and 500 Gs for 15 min once daily; rats in the sham-stimulated group were only inputted magnetic stimulator.Seven,14 and 21 d after the group division,the recovery of neurological function was evaluated by 18-point nerve dysfunction scoring method,and immunohistochemistry was used to detect the nestin and Brdu positive cells in the hippocampus of rats in each group.Results The neurological deficit scores in the 11 mHz ILF-TMS group were significantly higher than those in the sham-stimulated group and sham-operated group 14 and 21 d after group after dividing (P＜0.05).As compared with sham-stimulated group and sham-operated group,1 mHz ILF-TMS group had increased number of nestin positive cells,with significant difference on the 7th,14th and 21st d; there was no statistical significance in the number of Brdu positive cells between sham-stimulated group and 1 mHz ILF-TMS group (P＜0.05).Conclusions Brain injury can stimulate the expression of nestin and Brdu in a certain degree.Although 11 mHz ultra-low frequency transcranial magnetic stimulation can improve the expression of nestin more effectively,the function of promoting cell proliferation of Brdu-positive cells is not observed.
