Effect of Kv channel-interacting protein 1 over-expression on excitability of primary cultured hippocampal neurons
10.3760/cma.j.issn.1671-8925.2014.10.004
- VernacularTitle:过表达KCNIP1蛋白对原代培养海马神经元兴奋性的影响
- Author:
Meng LI
1
;
Linming ZHANG
;
Guoyao XU
;
Yonghong YI
;
Tao SU
Author Information
1. 广州医科大学附属第二医院神经科学研究所
- Keywords:
Kv channel-interacting protein 1;
Transfection;
Potassium current;
Cell excitability
- From:
Chinese Journal of Neuromedicine
2014;13(10):990-993
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Kv channel-interacting protein 1 (KCNIP1) over-expression on K+ currents and neuronal excitability in primary cultured hippocampal neurons.Methods Enhanced green fluorescent protein plasmids carried KCNIP1 (pEGFP-KCNIP1) were established; empty pEGFP vectors were used as controls; primary cultured hippocampal neurons were transfected with pEGFP-KCNIP1 and control vectors.Whole-cell patch clamp technique was used for electrophysiological recording.Results The cultured neurons transfected with pEGFP-KCNIP1 led to KCNIP1 over-expression.The amplitudes of A-type K+ currents in the KCNIP1-overexpress neurons were significantly higher than that in the control group ([0.96±0.17] nA vs.[0.72±0.09] nA,P＜0.05),while no significant difference was found between the component of steady-state outwards K+ currents and controls.Current clamp analysis revealed significantly decreased frequency of evoked discharges and subthreshold membrane potential oscillations,and statistically increased membrane resistance of the hippocampal neurons in the group of KCNIP1 over-expression as compared with those in the controls (P＜0.05).Conclusion Over-expression of KCNIP1 could inhibit neuronal discharges possibly via its potentiation on A-type K+ currents.
