Relation between SOX2 and miR-200b and their influences in clinical pathology and prognosis in gliomas
10.3760/cma.j.issn.1671-8925.2014.10.003
- VernacularTitle:SOX2和miR-200b对胶质瘤临床病理和预后的影响及其相互关系
- Author:
Chang CHAI
1
,
2
;
Laijun SONG
;
Shuangyin HAN
;
Bo YANG
Author Information
1. 450052郑州,郑州大学第一附属医院神经外科
2. 450003郑州,河南省眼科研究所、河南省立眼科医院、郑州大学人民医院中心实验室
- Keywords:
Glioma;
MiR-200b;
SOX2;
Prognosis
- From:
Chinese Journal of Neuromedicine
2014;13(10):984-989
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the relation between SOX2 and miR-200b and their influences in clinical pathology and prognosis of gliomas patients.Methods One hundred and twenty-three human glioma specimens,collected in our hospital from January 2001 to December 2005 and conformed by pathology,were chosen in our study; other 23 healthy brain tissues collected during intracranial decompression were used as controls.Real-time quantitative PCR (qRT-PCR) was employed to detect the miR200b expression; immunohistochemistry was used to detect the SOX2 expression.Relations between SOX2 and miR-200b in different grades of gliomas were analyzed; the correlations of clinical parameters with SOX2 and miR-200b expressions were analyzed; Cox proportional hazards regression model was used to analyze the influences of SOX2 and miR-200b expressions in survival times of the patients,and the survival curves of patients with different SOX2 and miR-200b expressions were compared.Results The higher the pathology grade,the lower the miR-200b expression; gliomas of grade Ⅲ and Ⅳ had SOX2 positive expression enjoyed significantly lower miR-200b expression than those had SOX2 negative expression (P＜0.05).The SOX2 and miR-200b expressions in gliomas of different pathology grades were significantly different (P＜0.05).Cox proportional hazards regression model indicated that miR-200b and SOX2 were the independent risk factors for prognosis of gliomas.In patients of grade Ⅲ and grade Ⅳ gliomas,significantly higher 5 years survival rate in patients with high miR200b expression was noted as compared with that with low miR200b expression (P＜0.05),and significantly higher 5 years survival rate in patients with negative SOX2 expression was noted as compared with those with positive SOX2 expression (P＜0.05).Conclusion The miR-200b and SOX2 expressions show difference only in poor differentiated tissues of grade Ⅲ and Ⅳ gliomas; the two have influence in the survival time.
