Effect of ultra short-term low-dose irradiation on invasive properties of glioma cells: an in vitro study
10.3760/cma.j.issn.1671-8925.2014.09.004
- VernacularTitle:超短期低剂量伽玛刀照射对胶质瘤细胞侵袭性的影响
- Author:
Yiguang LIN
1
;
Xiaomin LIU
;
Zhiyuan ZHANG
;
Yanhe LI
;
Dong LIU
;
Guokai WANG
;
Desheng XU
Author Information
1. 天津医科大学第二医院神经外科伽玛刀治疗中心
- Keywords:
Glioma;
Irradiation;
Gamma knife;
Low dose;
Ultra short-term;
Invasion
- From:
Chinese Journal of Neuromedicine
2014;13(9):881-884
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the effect of ultra short-term low-dose gamma knife radiation on invasive properties of glioma cells at the genetic level.Methods Malignant glioma cell lines U87 and U251 were treated with gamma knife radiation,respectively,with a marginal doses of 0,4,6,8 and 10 Gy (all to the 80％ isodose line).Real time-PCR was used to measure the mRNA expressions of AKT-1,AKT-2,β-catenin and TCF-4 at 30 min after radiation.Results The expressions of AKT-1,AKT-2,β-catenin,TCF-4 in glioma cell lines after low-dose gamma knife radiation with a marginal dose of 6-8 Gy were significantly increased as compared with those after 0 Gy (P＜0.05); reduced expressions ofAKT-1,AKT-2,β-catenin and TCF-4 after a marginal dose of 10 Gy were found as compared with those after 0 Gy (P＜0.05); the β-catenin and AKT-1 mRNA expressions in U87 cells and TCF-4,AKT-1 and AKT-2 mRNA expressions in U251 cells enjoyed the most obvious increase at a marginal dose of 6 Gy; the TCF-4 and AKT-2 mRNA expressions in U87 cells and β-catenin mRNA expression in U251 cells enjoyed the most obvious increase at a marginal dose of 8 Gy.Conclusion Low-dose gamma knife radiation (6-8 Gy) could increase the β-catenin,TCF-4,AKT-1 and AKT-2 expressions,while high dose (10 Gy) could inhibit these effects in a short time period of 30 min.
