Autologus peripheral blood stem transplantation without the cryopreseration in the treatment of the malignant hematologic pathology
- Author:
Be Van Tran
- Publication Type:Journal Article
- Keywords:
blood stem transplantation
- MeSH:
Transplantation;
blood;
Hematologic Diseases
- From:Journal of Vietnamese Medicine
1999;232(1):1-11
- CountryViet Nam
- Language:Vietnamese
-
Abstract:
We performed 10 cases of autologous peripheral blood stem cell transplantation without cryoprevervation in 11/1996 and 12/1997 12. Patients were diagnosed of AML, ALL, CML and non Hodgkin’s lymphoma. The median age of the patients was 34 years (range 18-43). Peripheral blood stem cell mobilizing regiments consisted of Daunorubicine (50 mg/ m2/d, d1-d5) cytarabine and etoposide in 3 CML patients and high dose cyclophosphamide in the seven other patients, followed by G-CSF starting when an ANC< 0.5x 109/l until the day before the last leukapheresis. When a WBC count reached 4x109/l leukapheresis was started and continued for 3 consecutive days, as soon as the third collection was finished Melphalan was given as a conditioning regimen, 42 hours after giving Melphalan, the collected PSCs which were preserved at +40C (90 hours after giving Melphalan, the collected PSCs which were preserved at + 40C were reinfused through a central Hickman’s catheter. GM-CSF was given during the nadir period until the an ANC > 1x109/l for 3 consecutive days. The median number of mononucleated cells and CD34+ cell were 5x108/kg respectively. All platelet count > 20 x 109/l was 13 days and 20 days following transplantation, all patients are still in CR and alive with the median follow-up of 8 months.
