Effect of low frequency stimulation in the pedunculopontine tegmental nucleus on gait and motor activity in hemiparkinsonisom rat models
10.3760/cma.j.issn.1671-8925.2014.06.004
- VernacularTitle:低频电刺激脚桥被盖核对帕金森病大鼠步态和运动能力的影响
- Author:
Yong YANG
1
;
Wangming ZHANG
;
Weixin ZHANG
;
Minjie LUO
;
Jun WANG
;
Ming ZHOU
;
Peng WENG
Author Information
1. 510280广州,南方医科大学珠江医院神经外科,广东神经外科研究所,广东省脑功能修复与再生重点实验室
- Keywords:
Pedunculopontine tegmental nucleus;
Low frequency stimulation;
Parkinson's disease;
Catwalk gait system;
Open field test
- From:
Chinese Journal of Neuromedicine
2014;13(6):552-557
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of low frequency stimulation (LFS) in the pedunculopontine tegmental nucleus (PPTg) on gait and movement ability in unilateral lesioned Parkinson's disease(PD) rats.Methods Thirty-two male SD rats were randomly assigned to control group (6-hydroxydopamine treatment,n=8) and experimental group (6-hydroxydopamine treatment combined with electrode implantation,n=24).All subjects were processed with injection of 6-hydroxydopamine in the fight medial forebrain bundle (MFB) and simultaneously the rats in the experimental group were received an electrode implantation into the ipsilateral PPTg.Catwalk gait and open field test data were acquired at 1 week before stereotactic surgery and 3 weeks after operation in the rats of control and experimental groups; the behavioral data of rats in the experimental group were recorded again 4 weeks after operation when they were under ongoing LFS-PPTg.Results Catwalk results showed that the postoperative couplings values (right front→left front,right front→left hind,right hind →left front,right hind →left hind) were significantly increased in all subjects as compared with the preoperative ones (P＜0.05); while couplings value in rats of the experimental group after LFS-PPTg significantly reduced as compared with that before LFS-PPTg (P＜0.05).The postoperative run speed variation statistically increased and postoperative cadence significantly decreased in all rats as compared with the preoperative ones (P＜0.05); the run speed variation significantly decreased and cadence obviously increased in experimental group after LFS-PPTg as compared with those before LFS-PPTg (P＜0.05).Open field test:the moving distance of all subjects decreased significantly after operation; the distance in rats of the experimental group after LFS-PPTg increased significantly as compared with that before LFS-PPTg.Conclusion LFS-PPTg can effectively improve gait function and motor activity of unilateral PD rat models.
