Single nucleotide polymorphisms in MicroRNA binding sites of susceptibility genes and association of oral contraceptive use with risk of stroke in Chinese women
10.3760/cma.j.issn.1671-8925.2014.05.012
- VernacularTitle:易感基因MicroRNA结合区位点多态性及使用口服避孕药与女性脑卒中发病风险的关联研究
- Author:
Tong CHEN
1
;
Ying LI
;
Chun WANG
;
Zhiming SUN
;
Jian ZHOU
;
Lei BA
;
Xiaoping HUANG
Author Information
1. 南京医科大学公共卫生学院流行病学和卫生统计学系
- Keywords:
Combined oral contraception;
MicroRNA binding site;
Single nucleotide polymorphism;
Stroke
- From:
Chinese Journal of Neuromedicine
2014;13(5):484-488
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the associations of stroke with oral contraception (COC) use,single nucleotide polymorphisms (SNPs) in miRNA binding sites of susceptibility genes and their joint effects in women.Methods Four hundred and fifty-three female patients with stroke,accepted COC or intrauterine device from two regions from July 1997 to June 2009,were chosen as stroke group,including 165 with hemorrhagic stroke,285 with infarct stroke and 3 with indeterminate stroke.The ageand region-matched controls (n=919) were recruited from the female cohort.The clinical data of these two groups were collected.Genotyping of 4 SNPs in miRNA binding sites in the reninangiotensin-aldosterone system (RAAS) genes was performed by polymerase chain reaction assay with Taqman probes.Unconditioned Logistic regression and dichotomy analyses were employed to analyze the influences of COC use and each genotype in stroke.Results As compared with the control group,the stroke group had significantly higher ratios of COC use,hypertension,hyperlipidemia and family history of stroke (P＜0.05); as compared with the wild-type homozygote rs5186AA,both AC genotype and combined genotype AC/CC of rs5186 were associated with a significant risk effect for hemorrhagic stroke (OR=1.83,95％CI:1.10-2.97;OR=1.74,95％CI:1.06-2.87).As compared with non-users without genetic variant,COC user without genetic variant increased the risk of hemorrhagic stroke by 0.71 fold (OR=1.71,95％CI:1.18-2.48),and COC use combined with genetic variant of rs5186 increased the risk of hemorrhagic stroke by 1.81 fold (OR=2.81,95％CI:1.45-5.64); as compared with non-users without genetic variant,COC user without genetic variant increased the stroke risk by 0.32 fold (OR=1.32,95％CI:1.04-1.68).Conclusion The variant of rs5186(A＞C) of angiotensin Ⅱ typer 1 receptor gene is positively associated with risk of hemorrhagic stroke,and COC use combined with this genetic variant significantly increases the risk of hemorrhagic stroke.
