Effect on transplantation of bone marrow mononuclear cells on neuron-like cell differentiation and glial cell-derived neurotrophic factor expression in rats with intracerebral hemorrhage
10.3760/cma.j.issn.1671-8925.2014.01.005
- VernacularTitle:骨髓单个核细胞移植对脑出血大鼠神经元样细胞分化和GDNF的影响
- Author:
Xueying ZHANG
1
;
Hong LU
;
Jianping WANG
;
Chao JIANG
;
Jun CHEN
;
Menghan WANG
Author Information
1. 郑州大学第一附属医院神经内科
- Keywords:
Intracerebral hemorrhage;
Bone marrow mononuclear cell;
Glial cell-derived neurotrophic factor
- From:
Chinese Journal of Neuromedicine
2014;13(1):22-25
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of transplantation of bone marrow mononuclear cells (BMMNCs) on neuron-like cell differentiation and glial cell-derived neurotrophie factor (GDNF) expression in rats with intracerebral hemorrhage (ICH).Methods ICH was induced by stereotactic injection of type Ⅳ collagenase into the striatum of rats,and the successful models were randomly divided into model group (ICH group) and BMMNCs transplantation group (BMMNCs group); BMMNCs group was injected with 1 mL cell suspension containing 3 ×107 cells marked with 5-Bromo-2-deoxyuridine (Brdu).The neurological functions of the rats were observed by modified neurological severity scale (mNSS) test 1,7,14 and 28 days after the injection; the GDNF expression in cerebral tissue was observed by Western blotting 1,7,14 days after the injection; double immunofluorescent labeling was utilized to trace the differentiation of BMMNCs in the rat brains 28 days after the injection.Results As compared with those in the ICH group,the mNSS scores in the BMMNCs group were significantly lower (P＜0.05) and the GDNF expression was significantly increased at all time points (P＜0.05); Brdu and neuron specific nuclear protein (NeuN) double-labeled positive cells were found in the hemorrhage focus in the BMMNCs group 28 d after injection.Conclusion BMMNCs transplantation can improve the neural behavioral function,increase the GDNF expression,and induce BMMNCs differentiating into neuron-like cells.
