Plasma miRNA-124 and miRNA-765 as biomarkers for severe traumatic brain injury
10.3760/cma.j.issn.1671-8925.2013.12.021
- VernacularTitle:血浆miRNA-124和miRNA-765作为重型颅脑创伤患者生物标记物的研究
- Author:
Ai-Lin LI
1
;
Qiong WANG
;
Da-Shi ZHI
Author Information
1. 300061,天津市环湖医院颅脑创伤中心
- Keywords:
Severe traumatic brain injury;
Plasma;
miRNA
- From:
Chinese Journal of Neuromedicine
2013;12(12):1283-1285
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the changes of plasma miRNA-124 and miRNA-765 levels in patients with severe traumatic brain injury (sTBI).Methods Thirty sTBI patients,admitted to our hospital from January 2009 to March 2010,and 24 healthy volunteers were included in the study.Quantitative RT-PCR was employed to determine the levels of miRNA-124 and miRNA-765 in the plasma and traumatic brain tissues at different time points after injury; receiver operating characteristic curve (ROC) was used to explore the potential of miRNA-124 and miRNA-765 as diagnostic TBI biomarkers.Results MiRNA-124 level dominantly increased 6 h after TBI and continued to rise 12 and 24 h,while miRNA-765 reached its peak level at 12 h after TBI in patients with sTBI,enjoying significant differences as compared with those in the matched healthy volunteers(t=2.41,P=0.001).miR-124 level (t=2.686,P=0.001) and miR-765 level (t=2.673,P=0.000) in the contusion and laceration tissues after sTBI predominantly increased as compared with those in the normal brain tissues surrounding to the contusion and laceration tissues,with significant difference.ROC curve analysis indicated that miR-124 and miR-765 were good diagnostic markers of sTBI (area under the curve:0.98 and 0.79).Conclusion Plasma-derived miR-124 and miR-765 change significantly,which could be employed as diagnostic markers for sTBI.
