Suppressive effect of miR-137 on proliferation and apoptosis of U87 glioma cells in vitro
10.3760/cma.j.issn.1671-8925.2013.12.007
- VernacularTitle:MiR-137对人脑胶质瘤细胞株U87细胞增殖和凋亡影响的体外研究
- Author:
Jun GUO
1
;
Ming LI
;
Zheng-Qiang WAN
;
Lin-Shan FU
;
Jin XU
;
Guan SUN
Author Information
1. 224001,盐城市第一人民医院神经外科
- Keywords:
MiR-137;
Proliferation;
Apoptosis;
Glioma
- From:
Chinese Journal of Neuromedicine
2013;12(12):1216-1219
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect ofmiR-137 on proliferation and apoptosis of U87 glioma cells.Methods The miR-137 mimics were transfected to human glioma cell line U87 as miR-137 transfection group; blank control group and negative control group were also employed in our study.The expression ofmiR-137 was identified by real-time polymerase chain reaction (PCR) after transfection.Methylthiazol tetrazoliu (MTT) assay,flow cytometry-Annexin V/PI assay and fluorescence microscope were employed to detect the cell proliferation and apoptosis.MiR-137 and its relative targeting protein expressions were detected by Westem blotting.Results MTT assay showed that the relative cell survival rates in the blank control group,negative control group and miR-137 transfection group were (105.16±8.57)％,(98.57±8.21)％ and (45.84±6.93)％,with significant differences (F=82.157,P=0.000).Annexin V/PI assay showed that the cell apoptosis rates in the blank control group,negative control group and miR-137 transfection group were (4.3±0.63)％,(4.7±0.77)％ and (16.6±1.98)％,with significant differences (F=63.837,P=0.000); and apoptotic body was detected by fluorescence microscope.Moreover,CDC42,pERK1/2,AKT and pAKT protein expression levels were inhibited after transfected by miR-137 mimics.Conclusion MiR-137 inhibits proliferation and induces apoptosis of U87 glioma cells,suggesting that miR-137 could be tumor suppressor gene in glioma.
