Effect of recombinant human granulocyte colony-stimulating factor on neural function in rats after intracerebral hemorrhage
10.3760/cma.j.issn.1671-8925.2013.09.006
- VernacularTitle:重组人粒细胞集落刺激因子对脑出血大鼠神经功能的影响
- Author:
Ming-Wu XIA
1
;
Hao ZHAO
;
Qian GUO
;
Yong QIN
;
Hao XU
;
Guang-Min HU
Author Information
1. 230011,合肥市第二人民医院神经内科
- Keywords:
Intracerebral hemorrhage;
Recombinant human granulocyte colony-stimulating factor;
Neurological function
- From:
Chinese Journal of Neuromedicine
2013;12(9):891-895
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of recombinant human granulocyte colony-stimulating factor (rhG-CSF) on neural function in rats after intracerebral hemorrhage.Methods Forty-eight SD rats were randomly divided into model group and rhG-CSF treatment group; ntracerebral hemorrhage models in the two groups were induced by rat brain stereotaxic atlas via injecting autologous incoagulable blood obtained from the tail of rats.Rats in the rhG-CSF treatment group were administered with rhG-CSF at a dosage of 60 μg/ (kg·d) by intraperitoneal injection 1 h after the inducement; rats in the model group was administered with the same amount of 0.9％ Nacl.On the 7th and 14th day of operation,the neurological severity scores of the rats in two groups were recorded and the number of peripheral blood CD34+ cells was detected with flow cytometry; MAP-2 protein expression in the surrounding tissue of hemorrhage area at the different time points was tested by immunohistochemisty.Results In the rhG-CSF treatment group,the neurological severity scores (0.407±0.057,0.649±0.014),level of CD34+blood cells (0.381％±0.054％,0.205％±0.088％) and MAP-2 protein expression level (0.205±0.088,0.281 ±0.054) were significantly higher than those in the model group (0.168±0.066,0.326±0.047;0.074％±0.028％,0.030％±0.057％; 0.080±0.017,0.124±0.028,P＜0.05).Conclusion The rhG-CSF could improve neural function of rats after intracerebral hemorrhage,which may play an important role in the process of reparation of nerve tissues and neural functional recovery after hemorrhage.
