Effects of eicosanoic acid on APP and BACE1 expressions in cortical neurons of primary cultured rats

VernacularTitle:二十烷酸对大鼠原代皮质神经元APP及BACE1表达的影响
Author: Yu ZHANG ¹ ; Lei YANG ; Na XU ; Ling-Ling FENG ; Ru-Ling SHI
Author Information

1. 新乡医学院生物化学与分子生物学教研室
Keywords: Saturated fatty acid; Beta-amyloid; Beta-amyloid precursor protein; Beta-site APP cleaving enzyme; Alzheimer's disease
From: Chinese Journal of Neuromedicine 2013;12(7):653-656
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect ofeicosanoic acid (EA) on β-amyloid precursor protein (APP) and β-site APP cleaving enzyme 1 (BACE1) expressions in cortical neurons of primary cultured rats.Methods Cortical neurons of primary cultured rats were divided randomly into EA treatment group (being administered with 40 μmol/L eicosanoic acid for 24 h) and control group (medium containing the same amount of solvent).The APP and BACE1 protein levels were detected by Westem blotting.The APP and BACE1 mRNA levels were detected by reverse transcription-polymerase chain reaction (RT-PCR).Results The levels of APP751+770 mRNA,APP protein and BACE1 protein in the EA treatment group were significantly higher than those in control group (P＜0.05).Conclusion The saturated fatty acids increase the APP and BACE1 expressions,which may play an important role in the pathogenesis of Alzheimer's disease.