Effects of poly-L-lysine of different molecular weights on growth of primary hippocampal neurons
10.3760/cma.j.issn.1671-8925.2013.07.001
- VernacularTitle:L-多聚赖氨酸对原代海马神经元生长影响
- Author:
Yan HE
1
;
Kang PENG
;
Ji-Yin ZHOU
;
Ming-Jin JIANG
Author Information
1. 南方医科大学中医药学院
- Keywords:
Neuron;
Hippocampus;
Primary culture;
Poly-L-lysine
- From:
Chinese Journal of Neuromedicine
2013;12(7):649-652
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of poly-L-lysine of different molecular weights on the growth of primary hippocampal neurons in neonatal rats.Methods The hippocampal neurons from neonatal rats were prepared with the method of extrusion by needle core; and then,the hippocampal neurons were divided into three groups and respectively planted in culture plates where coated poly-L-lysine of different molecular weights (no poly-L-lysine,70 000-150 000 and 150 000-300 000).The neurons were maintained in Neurobasal-A medium without fetal bovine serum.The neurons were viewed at different time points and indentified by neuron specificity enolization enzyme immunofluorescence staining; the purity of the cells was calculated and the effects of poly-L-Lysine of different molecular weights on the growth of cells were observed.Results The neurons attached to the culture plates 1 h after the plantation.Four d after the plantation,the neurons had shining body,integrity stucture,having 2-3 synapses.Eight d after the plantation,the neurons became mature; the axons of neurons interweaved into the net; the cells were identified as neurons with an average purity of (92.6± 4.62)％.All neurons without poly-L-lysine almost died; the cells in the plates of poly-L-lysine of 70 000-150 000 distributed uniformly.Conclusion Different molecular weights of poly-L-lysine can affect the neurons adhesion and behavior; poly-L-lysine with large molecular weight (150 000-300 000) is most suitable for neurons.
